Sprouting of substance P-expressing primary afferent central terminals and spinal micturition reflex NK1 receptor dependence after spinal cord injury.
Sprouting of substance P-expressing primary afferent central terminals and spinal micturition reflex NK1 receptor dependence after spinal cord injury.
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脊髓损伤后表达 P 物质的初级传入中枢末梢的萌芽和脊髓排尿反射 NK1 受体依赖性。
DOI：
10.1152/ajpregu.90653.2008
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Dolber,PaulC
中科院分区：
文献类型：
--
作者：
Zhang,Xiaoyang;Douglas,KristyL;Jin,Huixia;Eldaif,BassemM;Nassar,Rashid;Fraser,MatthewO;Dolber,PaulC
大鼠脊髓完全性损伤（SCI）后触发脊髓排尿反射的主要传入神经递质尚不清楚。物质P检测化学在骶副交感神经核显着高于12脊髓损伤大鼠（P= 0.008），表明P物质作为一个合理的候选人的初级传入神经递质。然后通过在另外14只完整大鼠和14只SCI大鼠中进行清醒膀胱测压，在L 6-S1鞘内给予L-733060（0.1-100 μg），测定速激肽NK 1受体拮抗剂L-733060对脊髓排尿反射的影响。L-733060在10 ~ 100 μg剂量范围内对正常大鼠无作用，但对14只SCI大鼠中的10只脊髓排尿反射有阻断作用，对另外4只大鼠中的2只排尿间期有延长作用。根据阶段性尿道外括约肌（EUS）活动的存在而区分的阶段性和非阶段性排尿收缩，存在于大多数SCI大鼠中。两种类型的收缩都被高剂量的L-733060阻断。有趣的是，在高剂量下，阶段性排尿收缩相对下降，以及非阶段性排尿收缩的收缩幅度下降。在其他方面，膀胱测压变量在很大程度上不受脊髓完整或SCI大鼠。L-733060在任何剂量下均不影响强直性EUS活动，除非脊髓排尿反射被阻断，强直性活动因此丧失。这些实验表明，速激肽对脊髓NK 1受体的作用在SCI大鼠的脊髓排尿反射中发挥着重要作用。
The primary afferent neurotransmitter triggering the spinal micturition reflex after complete spinal cord injury (SCI) in the rat is unknown. Substance P detected immunohistochemically in the sacral parasympathetic nucleus was significantly higher in 12 SCI rats than in 12 spinally intact rats (P= 0.008), suggesting substance P as a plausible candidate for the primary afferent neurotransmitter. The effects of the tachykinin NK1 receptor antagonist L-733060 on the spinal micturition reflex were then determined by performing conscious cystometry in an additional 14 intact rats and 14 SCI rats with L-733060 (0.1–100 μg) administered intrathecally at L6-S1. L-733060 was without effect in intact rats, but blocked the spinal micturition reflex in 10 of 14 SCI rats and increased the intermicturition interval in 2 of 4 others at doses ranging from 10 to 100 μg. Both phasic and nonphasic voiding contractions, differentiated according to the presence of phasic external urethral sphincter (EUS) activity, were present in most SCI rats. Both types of contractions were blocked by high doses of L-733060. Interestingly, there was a relative decline in phasic voiding contractions at high doses as well as a decline in contraction amplitude in nonphasic voiding contractions. In other respects, cystometric variables were largely unaffected in either spinally intact or SCI rats. L-733060 did not affect tonic EUS activity at any dose except when the spinal micturition reflex was blocked and tonic activity was consequently lost. These experiments show that tachykinin action at spinal NK1 receptors plays a major role in the spinal micturition reflex in SCI rats.
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