A Versatile and Robust Platform for the Scalable Manufacture of Biomimetic Nanovaccines.
A Versatile and Robust Platform for the Scalable Manufacture of Biomimetic Nanovaccines.
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DOI：
10.1002/advs.202002020
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Leong KW
中科院分区：
文献类型：
--
作者：
Hu H;Yang C;Zhang F;Li M;Tu Z;Mu L;Dawulieti J;Lao YH;Xiao Z;Yan H;Sun W;Shao D;Leong KW
仿生策略对于设计高效疫苗很有用。用细胞膜碎片修饰纳米颗粒佐剂作为抗原呈递来源就是一个例子，这是一种很有前景的策略。对于转化而言，一种标准化、一致且可扩展的用细胞膜包裹纳米佐剂的方法很重要。在此，展示了一种称为快速纳米复合（FNC）的湍流混合和自组装方法，它能够以可扩展的方式生产细胞膜包裹的纳米疫苗。通过使用十种不同的核心材料和多种细胞膜类型，展示了这种FNC技术相较于批量超声处理的广泛适用性。FNC生产的仿生纳米颗粒具有良好的胶体稳定性和较窄的颗粒多分散性，这表明与批量超声处理方法相比，其包裹更加均匀或更均匀。接着展示了一种由包裹B16 - F10癌细胞膜并负载佐剂CpG的介孔二氧化硅纳米颗粒组成的纳米疫苗的效力。FNC制备的纳米疫苗与抗CTLA - 4联合使用时，在淋巴结靶向、树突状细胞抗原呈递和T细胞免疫激活方面显示出效力，从而在黑色素瘤小鼠模型中产生预防和治疗效果。这项研究推动了一种由稳健且通用的纳米制造技术实现的仿生纳米疫苗的设计。
快速纳米复合（FNC）被用于以稳健、通用、可重复且可扩展的方式生产细胞膜仿生纳米治疗剂。使用FNC制备的癌细胞膜包裹、负载CpG的介孔二氧化硅纳米颗粒，相较于超声处理制备的同类产品，在淋巴结靶向、T细胞激活、抗癌免疫反应以及联合免疫治疗效果方面表现更优。
Biomimetic strategies are useful for designing potent vaccines. Decorating a nanoparticulate adjuvant with cell membrane fragments as the antigen‐presenting source exemplifies, such as a promising strategy. For translation, a standardizable, consistent, and scalable approach for coating nanoadjuvant with the cell membrane is important. Here a turbulent mixing and self‐assembly method called flash nanocomplexation (FNC) for producing cell membrane‐coated nanovaccines in a scalable manner is demonstrated. The broad applicability of this FNC technique compared with bulk‐sonication by using ten different core materials and multiple cell membrane types is shown. FNC‐produced biomimetic nanoparticles have promising colloidal stability and narrow particle polydispersity, indicating an equal or more homogeneous coating compared to the bulk‐sonication method. The potency of a nanovaccine comprised of B16‐F10 cancer cell membrane decorating mesoporous silica nanoparticles loaded with the adjuvant CpG is then demonstrated. The FNC‐fabricated nanovaccines when combined with anti‐CTLA‐4 show potency in lymph node targeting, DC antigen presentation, and T cell immune activation, leading to prophylactic and therapeutic efficacy in a melanoma mouse model. This study advances the design of a biomimetic nanovaccine enabled by a robust and versatile nanomanufacturing technique. Flash nanocomplexation (FNC) is exploited to produce cell membrane‐biomimetic nanotherapeutics in a robust, versatile, reproducible, and scalable manner. Cancer cell membrane‐coated, CpG‐loaded mesoporous silica nanoparticles fabricated using FNC exhibit better lymph node targeting, T‐cell activation, anticancer immunoresponse, and combined immunotherapeutic outcome than sonication‐produced counterparts.
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影响因子：
10.8
作者：
Gao W;Fang RH;Thamphiwatana S;Luk BT;Li J;Angsantikul P;Zhang Q;Hu CM;Zhang L
通讯作者：
Zhang L
可生物降解的纳米颗粒的临界尺寸极限，用于增强淋巴结运输和副型穿透性。
影响因子：
9.9
作者：
Howard GP;Verma G;Ke X;Thayer WM;Hamerly T;Baxter VK;Lee JE;Dinglasan RR;Mao HQ
通讯作者：
Mao HQ
影响因子：
14
作者：
Fang RH;Jiang Y;Fang JC;Zhang L
通讯作者：
Zhang L
影响因子：
4.7
作者：
Liu, Ying;Cheng, Chungyin;Fox, Rodney O.
通讯作者：
Fox, Rodney O.
影响因子：
4.2
作者：
Liu, Zhenping;Ramezani, Mahdi;Olsen, Michael G.
通讯作者：
Olsen, Michael G.