Phosphohexose mutase of Xanthomonas oryzae pv. oryzicola is negatively regulated by HrpG and HrpX, and required for the full virulence in rice
Phosphohexose mutase of Xanthomonas oryzae pv. oryzicola is negatively regulated by HrpG and HrpX, and required for the full virulence in rice
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米黄单胞菌磷酸己糖变位酶。
DOI：
10.1007/s10658-014-0469-7
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发表时间：
2014-06
影响因子：
1.8
通讯作者：
Liu Jianzhong
中科院分区：
文献类型：
--
作者：
Fang yuan;Liu Xia;Chen Gongyou;Liu Jianzhong
水稻黄单胞菌的基因组。oryzicola注释了一个未表征的基因XOC_3841，该菌株中只有一个ORF被注释编码磷酸己糖突变酶（XanA），该酶可将葡萄糖1-磷酸可逆地转化为葡萄糖6-磷酸，涉及碳代谢途径。然而，目前尚不清楚xana编码基因是否参与了X. oryzae pv的全部毒力。oryzicola。在这篇报道中，我们发现在独特的xanA中诱变，有效地导致病原体无法利用葡萄糖和半乳糖生长。葡萄糖、蔗糖、果糖、甘露糖或半乳糖强烈诱导xanA表达量比无糖NY培养基高至少3倍。有趣的是，xanA启动子区域包含一个不完善的PIP-box（植物诱导启动子）（TTCGC-N16-TTCGA），并且xanA的表达在水稻悬浮细胞中而不是在富营养（NB）培养基中诱导，并受到hrp关键调控因子HrpG和HrpX级联的负调控。更重要的是，xanA突变导致植物中细菌生长和毒力受损，降低了培养基中细菌的细胞活力和细胞外多糖（EPS）的产生。此外，由于trans中存在xanA， RΔxanA中提到的失去的性质完全恢复到野生型水平。这些结果表明，xanA是水稻X. oryzae pv产生EPS、细胞运动和完全毒力所必需的。oryzicola。
The genome of Xanthomonas oryzae pv. oryzicola annotates one uncharacterized gene, XOC_3841, only one ORF in this strain is annotated to encode Phosphohexose mutase (XanA), which reversibly converts glucose 1-phosphate to glucose 6-phosphate that implicates in the carbon metabolism pathways. However, it is unclear whether the XanA-coding gene is involved in the full virulence of X. oryzae pv. oryzicola. In this report, we showed that the mutagenesis in unique xanA, led the pathogen effectively to unable to utilize glucose and galactose for growth. The expression of xanA was strongly induced by glucose, sucrose, fructose, mannose or galactose at least 3 times higher than that by non-sugar NY medium. Intriguingly, xanA promoter region contains an imperfect PIP-box (plant-inducible promoter) (TTCGC-N16-TTCGA), and the expression of xanA was inducible in rice suspension cells rather than in a nutrient-rich (NB) medium and negatively regulated by a key hrp regulatory HrpG and HrpX cascade. More importantly, mutation in xanA resulted in impairment of bacterial growth and virulence in planta, and reduced bacterial cell motility and extracellular polysaccharides (EPS) production in media. In addition, the lost properties mentioned above in RΔxanA were completely restored to the wild-type level by the presence of xanA in trans. All these results suggest that xanA is required for EPS production, cell motility and the full virulence of X. oryzae pv. oryzicola.
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DOI：
10.1016/0140-6736(90)92435-k
发表时间：
1950-05
期刊：
The Yale Journal of Biology and Medicine
影响因子：
--
作者：
S. Eykyn
通讯作者：
S. Eykyn
影响因子：
1.9
作者：
A. Slusarenko;R. Fraser;L. C. Loon
通讯作者：
A. Slusarenko;R. Fraser;L. C. Loon
影响因子：
2.1
作者：
Furutani, A;Nakayama, T;Tsuge, S
通讯作者：
Tsuge, S
影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
HrpD6 在调节米黄单胞菌 hrp-hrc-hpa 基因中的新调控作用。
影响因子：
3.5
作者：
Li, Yu-Rong;Zou, Hua-Song;Chen, Gong-You
通讯作者：
Chen, Gong-You