The role of cholesterol in UV light B-induced apoptosis.
The role of cholesterol in UV light B-induced apoptosis.
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DOI：
10.1111/j.1751-1097.2011.01038.x
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发表时间：
2012-09
影响因子：
3.3
通讯作者：
Wu S
中科院分区：
文献类型：
--
作者：
George KS;Elyassaki W;Wu Q;Wu S
主要的脂筏成分，如胆固醇和神经酰胺的修饰，在调节各种刺激下的细胞程序性死亡中发挥作用。然而，胆固醇水平改变与紫外线B光诱导的细胞凋亡信号级联激活之间的关系尚未建立。在这份报告中，我们证明了UVB辐射后皮肤细胞膜筏中的胆固醇水平增加，从而导致Fas在筏子中聚集。利用连续速度浮选技术，我们发现经UVB照射后，Fas在人黑色素瘤M624细胞的脂筏中积聚。在脂筏组分中也检测到随后的盘状形成事件。甲基-β-环糊精耗尽胆固醇会减少Fas聚集，而超载则会增加。破坏脂筏还可以阻止Daxx从脂筏中的Fas解离，这伴随着UVB照射的人角质形成细胞HaCaT细胞的凋亡减少，但非凋亡性死亡增加。结果表明，位于皮肤细胞质膜中的胆固醇是形成脂筏结构域和激活UVB诱导的细胞凋亡所必需的。
Modification of major lipid raft components, such as cholesterol and ceramide, plays a role in regulation of programmed cell death under various stimuli. However, the relationship between cholesterol level modification and the activation of apoptotic signaling cascades upon ultraviolet B light has not been established. In this report, we demonstrate that upon UVB irradiation cholesterol levels in membrane rafts of skin cells increase, which leads to Fas aggregation in the rafts. Utilizing a continuous velocity floatation technique, we show that Fas accumulated in the lipid rafts of human melanoma M624 cells after UVB-irradiation. The subsequent events of DISC formation were also detected in the lipid raft fractions. Depletion of cholesterol by methyl-β-cyclodextrin reduces Fas aggregation, while overloading increases. Disruption of lipid rafts also prevents Daxx from dissociating from Fas in the lipid rafts, which is accompanied with a reduced apoptotic, but increased non-apoptotic death of UVB-irradiated human keratinocytes, HaCaT cells. Results indicate that cholesterol located in the plasma membrane of skin cells is required for lipid raft domain formation and activation of UVB-induced apoptosis.
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DOI：
10.1152/ajpgi.00082.2008
发表时间：
2008-11-01
影响因子：
4.5
作者：
Vassilieva, Elena V.;Gerner-Smidt, Kirsten;Nusrat, Asma
通讯作者：
Nusrat, Asma
影响因子：
3
作者：
PICARDO, M;ZOMPETTA, C;PROTA, G
通讯作者：
PROTA, G
影响因子：
3.3
作者：
Elyassaki, Walid;Wu, Shiyong
通讯作者：
Wu, Shiyong
影响因子：
3.6
作者：
Bang, B;Gniadecki, R;Gajkowska, B
通讯作者：
Gajkowska, B
影响因子：
1.5
作者：
Tsuda, Kayoko;Furuta, Nobumichi;Amano, Atsuo
通讯作者：
Amano, Atsuo