Nociceptor interleukin 10 receptor 1 is critical for muscle analgesia induced by repeated bouts of eccentric exercise in the rat.
Nociceptor interleukin 10 receptor 1 is critical for muscle analgesia induced by repeated bouts of eccentric exercise in the rat.
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DOI：
10.1097/j.pain.0000000000000936
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发表时间：
2017-08
期刊：
影响因子：
7.4
通讯作者：
Levine JD
中科院分区：
文献类型：
--
作者：
Alvarez P;Bogen O;Green PG;Levine JD
延迟性肌肉酸痛通常在剧烈或不习惯的离心运动后观察到。恢复后不久，在重复离心运动中观察到钝性肌肉酸痛，这种现象称为重复回合效应（RBE）。虽然有规律的体力活动减少肌肉痛觉过敏，可能是因为骨骼肌中抗炎细胞因子白细胞介素-10（IL-10）的产生增加，但IL-10是否也有助于RBE的抗伤害效应尚不清楚。此外，IL-10是否通过作用于肌肉伤害感受器来减弱肌肉痛觉过敏仍有待确定。在这里，我们探讨了这一假设，即在RBE中观察到的钝性肌肉伤害感受依赖于IL-10的局部作用，作用于肌肉伤害感受器表达的IL-10受体1（IL-10 R1）。结果显示，在第二轮离心运动后，大鼠表现出减少的肌肉痛觉过敏，指示RBE，并且在运动的腓肠肌中IL-10的表达增加。尽管鞘内注射反义寡核苷酸敲低腓肠肌伤害性感受器中的IL-10 R1蛋白并没有改变幼稚大鼠的伤害性感受阈值，但它揭示了12天前离心运动大鼠的潜伏性肌肉痛觉过敏。此外，反义还防止了第二轮离心运动后观察到的肌肉痛觉过敏的减少。这些数据表明，偏心运动后的伤害性阈值的恢复和RBE诱导的镇痛依赖于IL-10的局部作用，作用于肌肉伤害性感受器中的经典受体。
Delayed-onset muscle soreness is typically observed after strenuous or unaccustomed eccentric exercise. Soon after recovery, blunted muscle soreness is observed on repeated eccentric exercise, a phenomenon known as repeated bout effect (RBE). Although regular physical activity decreases muscle hyperalgesia, likely because of increased production of the anti-inflammatory cytokine interleukin-10 (IL-10) in the skeletal muscle, whether IL-10 also contributes to the antinociceptive effect of RBE is unknown. Furthermore, whether IL-10 attenuates muscle hyperalgesia by acting on muscle nociceptors remains to be established. Here, we explored the hypothesis that blunted muscle nociception observed in RBE depends on a local effect of IL-10, acting on IL-10 receptor 1 (IL-10R1) expressed by muscle nociceptors. Results show that after a second bout of eccentric exercise, rats exhibited decreased muscle hyperalgesia, indicative of RBE, and increased expression of IL-10 in the exercised gastrocnemius muscle. Although knockdown of IL-10R1 protein in nociceptors innervating the gastrocnemius muscle by intrathecal antisense oligodeoxynucleotide did not change nociceptive threshold in naive rats, it unveiled latent muscle hyperalgesia in rats submitted to eccentric exercise 12 days ago. Furthermore, antisense also prevented the reduction of muscle hyperalgesia observed after a second bout of eccentric exercise. These data indicate that recovery of nociceptive threshold after eccentric exercise and RBE-induced analgesia depend on a local effect of IL-10, acting on its canonical receptor in muscle nociceptors.
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影响因子：
7.4
作者：
Leung A;Gregory NS;Allen LH;Sluka KA
通讯作者：
Sluka KA
影响因子：
24.5
作者：
Hughes, Patrick A.;Harrington, Andrea M.;Brierley, Stuart M.
通讯作者：
Brierley, Stuart M.
影响因子：
3.3
作者：
ARMSTRONG, RB;OGILVIE, RW;SCHWANE, JA
通讯作者：
SCHWANE, JA
影响因子：
15.1
作者：
Ledeboer, Annemarie;Jekich, Brian M.;Watkins, Linda R.
通讯作者：
Watkins, Linda R.
影响因子：
7.4
作者：
Hendrich J;Alvarez P;Joseph EK;Chen X;Bogen O;Levine JD
通讯作者：
Levine JD