The molecular regulatory mechanisms of the bacteria involved in serpentine weathering coupled with carbonation
The molecular regulatory mechanisms of the bacteria involved in serpentine weathering coupled with carbonation
复制标题
细菌参与蛇纹石风化与碳化作用的分子调控机制
DOI：
10.1016/j.chemgeo.2021.120069
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发表时间：
2021-03
期刊：
影响因子：
3.9
通讯作者：
Lian B
中科院分区：
文献类型：
--
作者：
Liu HL;Liu XR;Li XF;Fu ZY;Lian B
开发一种有效的生物技术方法来减少二氧化碳的持续排放已经引起了广泛的关注。蛇纹石生物风化与碳酸化耦合的研究是当前研究的热点。为了研究枯草芽孢杆菌的分子机制，我们在无Mg2+的培养基中添加蛇纹石培养枯草芽孢杆菌。根据风化蛇纹石的有机质组成和特征，采用定量蛋白质组学技术阐明蛋白质在耦合过程中的作用。电化学分析和蒸汽扩散结晶实验揭示了分子机理。结果表明，氧化还原酶的分泌促进了蛇纹石的转化，并驱动有机酸的合成，加速了蛇纹石的风化。在蛇纹岩风化后期即蛇纹岩碳酸化阶段，次生矿物表征表明形成鸟状石和无定形碳酸盐，并伴有一定的有机质。蛇纹石碳酸化阶段，微环境中过量的Ni驱动N代谢，促进pH升高，导致蛇纹石碳酸化。本研究丰富了我们在蛋白质水平上对蛇纹石生物风化与碳酸化耦合的分子机制的认识。
Development of an effective biotechnological method of reducing the continuous emission of CO2has caused wide public concern. Research on serpentine bio-weathering coupled with carbonation is a current focus of interest. To investigate the molecular mechanisms,Bacillus subtiliswas cultured in the Mg2+-free medium with the addition of serpentine. Based on the composition of organic matter and the characterization of the weathered serpentine, quantitative proteomics technology was adopted to elucidate the roles of proteins played in the coupled process. Electrochemical analysis and vapor diffusion crystallization experiments were also performed to reveal the molecular mechanisms. The results demonstrated that more oxidoreductases were secreted to facilitate the transformation of serpentine, and drive the synthesis of organic acids to accelerate the weathering of serpentine. In the late stage of serpentine weathering, that is, the serpentine carbonation stage, the characterization of the secondary minerals showed that struvite and amorphous carbonate had been formed with some organic matter. In serpentine carbonation stage, excessive Ni in the microenvironment drove N metabolism to facilitate the elevation of pH, resulting in the serpentine carbonation. This study enriched our understanding of the molecular mechanisms at protein-level in serpentine bio-weathering coupled with carbonation.
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