Data-driven design of a multiplexed, peptide-sensitized transistor to detect breath VOC markers of COVID-19.
Data-driven design of a multiplexed, peptide-sensitized transistor to detect breath VOC markers of COVID-19.
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DOI：
10.1016/j.bios.2023.115237
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发表时间：
2023-06-01
影响因子：
12.6
通讯作者：
中科院分区：
文献类型：
--
作者：
人类呼出的气体含有丰富的挥发性有机化合物(VOCs)混合物，其浓度会随着疾病或其他应激源的变化而变化。使用模拟气味结合蛋白(OBP)和机器学习方法，我们设计了一种复合的短VOC和碳结合多肽探针，可以检测到特征的VOC指纹。具体地说，我们以紧凑的分子传感器阵列中的与新冠肺炎相关的VOC为目标，该阵列可将蒸汽成分直接转换为多通道电信号。通过与蛋白质数据库结构的多序列比对，从选定的OBP中获得了快速合成的嵌合VOC和固体结合肽。利用表面等离子体共振光谱和石英晶体微天平验证了选择性多肽与目标VOCs和传感器表面的结合。VOC传感是通过多肽敏化、暴露沟道的碳纳米管晶体管来实现的。数据到设备管道使开发用于非侵入性监测、疾病诊断和环境暴露评估的新型设备成为可能。
Exhaled human breath contains a rich mixture of volatile organic compounds (VOCs) whose concentration can vary in response to disease or other stressors. Using simulated odorant-binding proteins (OBPs) and machine learning methods, we designed a multiplex of short VOC- and carbon-binding peptide probes that detect a characteristic “VOC fingerprint”. Specifically, we target VOCs associated with COVID-19 in a compact, molecular sensor array that directly transduces vapor composition into multi-channel electrical signals. Rapidly synthesizable, chimeric VOC- and solid-binding peptides were derived from selected OBPs using multi-sequence alignment with protein database structures. Selective peptide binding to targeted VOCs and sensor surfaces was validated using surface plasmon resonance spectroscopy and quartz crystal microbalance. VOC sensing was demonstrated by peptide-sensitized, exposed-channel carbon nanotube transistors. The data-to-device pipeline enables the development of novel devices for non-invasive monitoring, diagnostics of diseases, and environmental exposure assessment.
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DOI：
10.1097/jto.0b013e318233d80f
发表时间：
2012-01
期刊：
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
影响因子：
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作者：
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影响因子：
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DOI：
10.1073/pnas.0307882100
发表时间：
2004-02-24
影响因子：
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通讯作者：
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DOI：
10.1016/0305-0491(95)00019-5
发表时间：
1995-07-01
影响因子：
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