Bone marrow pro‐T and pro‐B lymphocyte clones express functional receptors for interleukin (IL) 3 and IL4/BSF‐1 and nonfunctional receptors for IL2-猫眼课题宝

骨髓 pro-T 和 pro-B 淋巴细胞克隆表达白细胞介素 (IL) 3 和 IL4/BSF-1 的功能性受体以及 IL2 的非功能性受体

DOI：

10.1002/eji.1830170211

发表时间：

1987

期刊：

European Journal of Immunology

影响因子：

5.4

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

P. Sideras;R. Palacios

结果表明，具有前T淋巴细胞特性的C4-77和C4-86骨髓克隆和具有前B淋巴细胞特性的BC/BM11和CB/Bm7克隆在重组白细胞介素4(RIL4)/BSF-1和IL3中生长，而在rIL2中不生长。细胞对rIL4/bsf-1的增殖反应总是低于∼对IL3的反应的50%。针对IL3敏感的小鼠细胞的CC11单抗不影响rIL4/BSF-1的作用，但能抑制IL3对克隆的作用。抗IL-2受体的PC61单抗对rIL-4/BSF-1或IL-3诱导的反应均无影响。通过免疫荧光染色和流式细胞仪检测，4个克隆均携带CC11单抗和PC61单抗识别的糖蛋白。我们得出结论：PRO-T和PRO-B克隆表达IL3和rIL4/BSF-1的功能性受体和IL2的非功能性受体，rIL4/BSF-1通过IL3和IL2非依赖的途径促进这些克隆的生长，并讨论了这些发现的可能的生物学意义。

It is shown here that the C4‐77 and C4‐86 bone marrow clones with properties of pro‐T lymphocytes and the Bc/Bm 11 and CB/Bm7 clones with characteristics of pro‐B lymphocytes grow in recombinant interleukin 4 (rIL4)/BSF‐1 and IL3, but not in rIL2. The proliferative cell responses to rIL4/BSF‐1 were always less that ∼ 50% of those achieved by the clones in response to IL3. The CC11 monoclonal antibody (mAb) specific for IL3‐sensitive mouse cells did not affect the action of rIL4/BSF‐1, but it did inhibit the action of IL3 on the clones. The PC61 mAb against IL2 receptors had no effect on either rIL4/BSF‐1‐ or IL3‐driven responses. All four clones carry on the cell membrane the glycoproteins recognized by the CC11 mAb and by the PC61 mAb as assessed by immunofluorescence staining and flow cytometry. We conclude that the pro‐T and the pro‐B clones express functional receptors for IL3 and rIL4/BSF‐1 and nonfunctional receptors for IL2, that rIL4/BSF‐1 promotes growth of these clones via an IL3‐ and IL2‐independent pathway and discuss the possible biological significance of these findings.

Three distinct signals can induce class II gene expression in a murine pre-B-cell line.