Metabolite profiling identifies candidate markers reflecting the clinical adaptations associated with Roux-en-Y gastric bypass surgery.
Metabolite profiling identifies candidate markers reflecting the clinical adaptations associated with Roux-en-Y gastric bypass surgery.
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DOI：
10.1371/journal.pone.0007905
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发表时间：
2009-11-19
期刊：
影响因子：
3.7
通讯作者：
Clément K
中科院分区：
文献类型：
--
作者：
Mutch DM;Fuhrmann JC;Rein D;Wiemer JC;Bouillot JL;Poitou C;Clément K
Roux-en-Y胃旁路手术(RYGB)与减肥、改善胰岛素敏感性和血糖稳态以及减少糖尿病和冠心病等并存疾病有关。为了进一步了解与RYGB手术相关的众多代谢适应，我们对胃旁路手术前后的血清代谢物进行了分析，并结合临床数据进行了综合代谢物的变化。分别于治疗前、治疗后3个月和6个月(n = 14；BMI = 4 6.2±1.7)用气相色谱-液质联用仪检测血清代谢产物。受试者术后体重相关参数减少，胰岛素敏感性改善。在RYGB(p<；0.05)后的前3个月内，测量的代谢物中有83种(48%)的丰度发生了显著变化，包括鞘氨醇、不饱和脂肪酸和支链氨基酸。将受试者分为肥胖组(n = 9)和肥胖/糖尿病组(n = 5)，确定了在所有时间点一致不同的8种代谢物，它们的血清水平随着RYGB的变化而变化：天冬酰胺、溶血磷脂酰胆碱(C18：2)、神经酸(C24：1)、对甲酚硫酸盐、乳酸、番茄红素、葡萄糖和甘露糖。上述代谢物的变化与体重指数(BMI)和胰岛素抵抗估计(HOMA-IR)的临床数据相结合。其中神经酸与HOMA-IR呈显著负相关(p = 0.001，R = −0.55)。RYGB术后病态肥胖受试者的全球代谢物分析提供了与该外科手术相关的显著代谢改变的新信息。将临床测量与代谢组学数据相结合，能够确定反映RYGB后代谢适应的标记物。
Roux-en-Y gastric bypass (RYGB) surgery is associated with weight loss, improved insulin sensitivity and glucose homeostasis, and a reduction in co-morbidities such as diabetes and coronary heart disease. To generate further insight into the numerous metabolic adaptations associated with RYGB surgery, we profiled serum metabolites before and after gastric bypass surgery and integrated metabolite changes with clinical data. Serum metabolites were detected by gas and liquid chromatography-coupled mass spectrometry before, and 3 and 6 months after RYGB in morbidly obese female subjects (n = 14; BMI = 46.2±1.7). Subjects showed decreases in weight-related parameters and improvements in insulin sensitivity post surgery. The abundance of 48% (83 of 172) of the measured metabolites changed significantly within the first 3 months post RYGB (p<0.05), including sphingosines, unsaturated fatty acids, and branched chain amino acids. Dividing subjects into obese (n = 9) and obese/diabetic (n = 5) groups identified 8 metabolites that differed consistently at all time points and whose serum levels changed following RYGB: asparagine, lysophosphatidylcholine (C18:2), nervonic (C24:1) acid, p-Cresol sulfate, lactate, lycopene, glucose, and mannose. Changes in the aforementioned metabolites were integrated with clinical data for body mass index (BMI) and estimates for insulin resistance (HOMA-IR). Of these, nervonic acid was significantly and negatively correlated with HOMA-IR (p = 0.001, R = −0.55). Global metabolite profiling in morbidly obese subjects after RYGB has provided new information regarding the considerable metabolic alterations associated with this surgical procedure. Integrating clinical measurements with metabolomics data is capable of identifying markers that reflect the metabolic adaptations following RYGB.
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影响因子：
12.3
作者：
Mutch DM;O'Maille G;Wikoff WR;Wiedmer T;Sims PJ;Siuzdak G
通讯作者：
Siuzdak G
影响因子：
2.9
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影响因子：
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通讯作者：
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
1.5
作者：
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通讯作者：
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