Distribution of the protein kinase C substrates MARCKS and MRP in the postnatal developing rat brain
Distribution of the protein kinase C substrates MARCKS and MRP in the postnatal developing rat brain
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蛋白激酶C底物MARCKS和MRP在出生后发育中的大鼠大脑中的分布
DOI：
10.1002/(sici)1096-9861(19980803)397:3
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发表时间：
1998
影响因子：
2.5
通讯作者：
R. Lenox
中科院分区：
文献类型：
--
作者：
Robert K. McNamara;R. Lenox
肉豆浆酰化的富丙氨酸C激酶底物（MARCKS）和MARCKS相关蛋白（MRP）都是膜相关的磷酸化蛋白，它们以蛋白激酶C磷酸化依赖的方式与钙调蛋白和丝状肌动蛋白相互作用。在本研究中，我们采用定量原位杂交技术检测了出生后(P)大鼠脑（出生后1,7,14,21和90天）中MARCKS和MRP基因的表达。在P1时，MRP在新皮质、纹状体、丘脑、小脑皮质和海马（CA1 - ca3、门部和颗粒细胞层）中表达量高，但在脑干中表达量低，在P7和P14之间，除海马CA1和颗粒细胞层外，这些区域的MRP表达均显著下降。在P14和P21之间，MRP表达在白质区域增加，包括胼胝体、穹窿和小脑深部白质。在P90（成人）时，MRP在嗅球、内侧束、海马CA1、颗粒细胞层内三分之二、颞皮质和内嗅皮质、胼胝体和穹窿、小脑白质中仍然强烈表达。在P1时，MARCKS在除脑干外的大多数脑区强烈表达，随后在P14时逐渐下降到接近成人水平。P14 ~ P21期间，MARCKS在海马门部的表达逐渐下降，在海马CA1、CA3和颗粒细胞层的表达仍然升高，在胼胝体和穹窿的表达急剧增加。P90时，MARCKS在海马CA3和脑门的表达下降，在海马CA1和颗粒细胞层、嗅球区域、内侧缰、颞叶皮层、小脑颗粒细胞和浦肯野细胞中表达强烈。MARCKS和MRP在发生神经元增殖、迁移和神经突生长的区域的表达表明在这些发育事件中起共同作用，而在发育期间和成年大脑中的表达差异提供了差异调节的证据。中华神经科杂志，1998,27(2):357 - 357。©1998 Wiley‐Liss, Inc。
The myristoylated alanine‐rich C kinase substrate (MARCKS) and MARCKS‐related protein (MRP) are both membrane‐associated phosphoproteins that interact with calmodulin and filamentous actin in a protein kinase C phosphorylation‐dependent manner. In the present study, we examined MARCKS and MRP gene expression in the postnatal (P) rat brain (1, 7, 14, 21, and 90 days after birth) by using quantitative in situ hybridization. At P1, MRP expression was high in neocortex, striatum, thalamus, cerebellar cortex, and hippocampus (CA1–CA3, hilus, and granule cell layer) but low in brainstem and, between P7 and P14, exhibited a dramatic decline in each of these regions except hippocampal CA1 and granule cell layers. Between P14 and P21, MRP expression increased in white matter regions including the corpus callosum, fimbria/fornix, and cerebellar deep white matter. At P90 (adult), MRP remained strongly expressed in the olfactory bulb, medial habenula, hippocampal CA1, and the inner two‐thirds of granule cell layer, temporal, and entorhinal cortices, the corpus callosum and fimbria/fornix, and cerebellar white matter. At P1, MARCKS was strongly expressed in the majority of brain regions except the brainstem, which subsequently declined gradually to approximate adult levels by P14. Between P14 and P21, MARCKS expression declined gradually in the hilus, remained elevated in hippocampal CA1, CA3, and granule cell layers, and increased dramatically in the corpus callosum and fimbria/fornix. At P90, MARCKS expression declined in hippocampal CA3 and hilus and remained strongly expressed in hippocampal CA1 and granule cell layers, regions of the olfactory bulb, the medial habenula, temporal cortex, and cerebellar granule and Purkinje cells. Expression of both MARCKS and MRP in regions undergoing neuronal proliferation, migration, and neurite outgrowth suggest a common role in these developmental events, whereas differences in expression during development and in the adult brain provide evidence of differential regulation. J. Comp. Neurol. 397:337–356, 1998. © 1998 Wiley‐Liss, Inc.
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磷酸化、高离子强度和钙调蛋白可逆转 MARCKS 与磷脂囊泡的结合。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kim,J;Shishido,T;Jiang,X;Aderem,A;McLaughlin,S
通讯作者：
McLaughlin,S
DOI：
10.1073/pnas.79.17.5249
发表时间：
1982-09
影响因子：
11.1
作者：
W. Wu;S. Walaas;A. Nairn;P. Greengard
通讯作者：
W. Wu;S. Walaas;A. Nairn;P. Greengard
DOI：
10.1073/pnas.93.13.6275
发表时间：
1996-06-25
影响因子：
11.1
作者：
Chen, JM;Chang, S;Aderem, A
通讯作者：
Aderem, A
分离神经末梢中 87-kDa 蛋白（一种主要蛋白激酶 C 底物）的磷酸化和相关易位。
DOI：
10.1073/pnas.86.7.2253
发表时间：
1989
影响因子：
11.1
作者：
Wang,JK;Walaas,SI;Sihra,TS;Aderem,A;Greengard,P
通讯作者：
Greengard,P
大鼠脑中磷脂敏感性 Ca2+ 依赖性蛋白激酶及其底物以及磷蛋白磷酸酶的发育研究。
DOI：
10.1073/pnas.81.10.3143
发表时间：
1984
影响因子：
11.1
作者：
Turner,RS;Raynor,RL;Mazzei,GJ;Girard,PR;Kuo,JF
通讯作者：
Kuo,JF