Demonstration of receptor-mediated chemotaxis by human spermatozoa. A novel quantitative bioassay.
Demonstration of receptor-mediated chemotaxis by human spermatozoa. A novel quantitative bioassay.
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人类精子受体介导的趋化性的演示。
DOI：
10.1016/0014-4827(85)90506-3
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发表时间：
1985
影响因子：
3.7
通讯作者：
C. Pert
中科院分区：
文献类型：
--
作者：
L. Gnessi;M. Ruff;F. Fraioli;C. Pert
描述了一种新的体外技术，用于测量人精子可溶性物质的趋化活性。这项新的生物测定表明，合成的趋化肽-甲酰基- metLeuPhe对人类精子具有有效的、特异性的（即受体介导的）趋化作用，其ec50为3.2 × 10−10M。用9 -甲酰基化肽类似物对人精子进行的定量趋化研究表明，与兔中性粒细胞结合和趋化研究中获得的肽效价的等级顺序没有区别（p<0.001）。的竞争对手中国银行(t-butoxycarbonyl)板式换热器列伊板式换热器列伊法10−6 m,完全抑制趋化作用引起f-Met列伊法10−9 m,能够改变一个数量级的摩尔浓度要求f-Met列伊板式换热器板式换热器和f-Met列伊板式换热器引起最大反应。n -甲酰化肽作为精子趋化剂的能力揭示了与结合、趋化性和溶酶体酶释放的高度相关性，这些酶体酶释放之前被用来定义中性粒细胞趋化受体。这是首次明确证明物质对人类雄性配子具有受体介导的趋化作用，这表明人类精子可能确实有能力对适当的环境信号作出趋化反应。
A novel in vitro technique is described for measuring the chemotactic activity of soluble substances for human spermatozoa. This new bioassay has demonstrated that the synthetic chemotactic peptideN-formyl-MetLeuPhe elicits a potent, specific (i.e., receptor-mediated) chemotactic effect on human spermatozoa with an EC50of 3.2 × 10−10M. Quantitative chemotactic studies on human spermatozoa with nineN-formylated-peptide analogs have shown a rank order of peptide potency indistinguishable (p<0.001) from that obtained in binding and chemotactic studies with rabbit neutrophils. The competitive antagonist Boc (t-butoxycarbonyl)PheLeuPheLeuPhe, 10−6M, completely inhibited the chemotaxis elicited by f-MetLeuPhe, 10−9M, and was able to shift by one order of magnitude the molar concentration required by f-MetLeuPhePhe and f-MetLeuPhe to elicit the maximal response. The ability ofN-formylated peptides to function as sperm chemoattractants reveals a high degree of correlation with binding, chemotaxis, and lysosomal enzyme release previously employed to define the neutrophil chemotactic receptor. This first unequivocal demonstration of substances having a receptor-mediated chemotactic effect for human male gametes suggests that human spermatozoa may indeed have the ability to respond chemotactically to appropriate environmental signals.
体外牛卵丘复合体刺激牛尾部附睾精子向前运动。
DOI：
10.1016/s0006-291x(83)80002-3
发表时间：
1983
影响因子：
3.1
作者：
Bradley,MP;Garbers,DL
通讯作者：
Garbers,DL