Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila.
Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila.
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DOI：
10.1083/jcb.149.5.1097
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发表时间：
2000-05-29
期刊：
影响因子：
--
通讯作者：
Gaertig J
中科院分区：
文献类型：
--
作者：
Xia L;Hai B;Gao Y;Burnette D;Thazhath R;Duan J;Bré MH;Levilliers N;Gorovsky MA;Gaertig J
We analyzed the role of tubulin polyglycylation in Tetrahymena thermophila using in vivo mutagenesis and immunochemical analysis with modification-specific antibodies. Three and five polyglycylation sites were identified at glutamic acids near the COOH termini of α- and β-tubulin, respectively. Mutants lacking all polyglycylation sites on α-tubulin have normal phenotype, whereas similar sites on β-tubulin are essential. A viable mutant with three mutated sites in β-tubulin showed reduced tubulin glycylation, slow growth and motility, and defects in cytokinesis. Cells in which all five polyglycylation sites on β-tubulin were mutated were viable if they were cotransformed with an α-tubulin gene whose COOH terminus was replaced by the wild-type COOH terminus of β-tubulin. In this double mutant, β-tubulin lacked detectable polyglycylation, while the α-β tubulin chimera was hyperglycylated compared with α-tubulin in wild-type cells. Thus, the essential function of polyglycylation of the COOH terminus of β-tubulin can be transferred to α-tubulin, indicating it is the total amount of polyglycylation on both α- and β-tubulin that is essential for survival.
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在嗜热四膜虫中，赖氨酸40在α-微管蛋白中的乙酰化并不是必需的。
影响因子：
7.8
作者：
GAERTIG, J;CRUZ, MA;BOWEN, J;GU, L;PENNOCK, DG;GOROVSKY, MA
通讯作者：
GOROVSKY, MA
DOI：
10.1073/pnas.94.4.1310
发表时间：
1997-02-18
影响因子：
11.1
作者：
Hai, B;Gorovsky, MA
通讯作者：
Gorovsky, MA
影响因子：
--
作者：
GAERTIG, J;THATCHER, TH;GOROVSKY, MA
通讯作者：
GOROVSKY, MA
影响因子：
3.3
作者：
Bré, MH;Redeker, V;Levilliers, N
通讯作者：
Levilliers, N
影响因子：
56.9
作者：
EDDE, B;ROSSIER, J;DENOULET, P
通讯作者：
DENOULET, P