Phenotypic and Functional Characterization of Double Negative B Cells in the Blood of Individuals With Obesity.
Phenotypic and Functional Characterization of Double Negative B Cells in the Blood of Individuals With Obesity.
复制标题
肥胖患者血液中双阴性B细胞的表型和功能特征。
DOI：
10.3389/fimmu.2021.616650
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发表时间：
2021
影响因子：
7.3
通讯作者：
Blomberg BB
中科院分区：
文献类型：
--
作者：
Frasca D;Diaz A;Romero M;Blomberg BB
我们之前已经证明，肥胖与具有抗自身反应性的 IgG 抗体分泌增加有关。在本文中，我们确认并扩展了我们之前的发现。我们发现，肥胖个体的血浆富含自身免疫抗体，其水平与双阴性 (DN) B 细胞亚群（最促炎的 B 细胞亚群）的血液频率呈正相关。我们还发现，与瘦人相比，肥胖者血液中的 DN B 细胞显着增加，其特点是免疫激活标记物和转录因子 T-bet 表达较高，这两者都与自身免疫相关。从外周 B 细胞池中去除 DN B 细胞可显着降低抗自身 IgG 抗体的体外分泌。这些结果共同证实了 DN B 细胞在肥胖个体分泌抗自身 IgG 抗体中的关键作用。
We have previously shown that obesity is associated with increased secretion of IgG antibodies with anti-self-reactivity. In this paper, we confirm and extend our previous findings. We show that the plasma of individuals with obesity is enriched in autoimmune antibodies whose levels are positively associated with blood frequencies of the subset of Double Negative (DN) B cells, which is the most pro-inflammatory B cell subset. We also show that DN B cells, significantly increased in the blood of obese versus lean individuals, are characterized by higher expression of immune activation markers and of the transcription factor T-bet, both associated with autoimmunity. The removal of DN B cells from the peripheral B cell pool significantly decreases in vitro secretion of anti-self IgG antibodies. These results altogether confirm the crucial role of DN B cells in the secretion of anti-self IgG antibodies in individuals with obesity.
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影响因子：
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