PhMYB4 fine-tunes the floral volatile signature of Petunia x hybrida through PhC4H.
PhMYB4 fine-tunes the floral volatile signature of Petunia x hybrida through PhC4H.
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DOI：
10.1093/jxb/erq342
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发表时间：
2011-01
影响因子：
6.9
通讯作者：
Clark DG
中科院分区：
文献类型：
--
作者：
Colquhoun TA;Kim JY;Wedde AE;Levin LA;Schmitt KC;Schuurink RC;Clark DG
矮牵牛花挥发性苯类化合物（FVBP）的合成在分子、代谢和生化水平上受到空间、发育和日常调控。已显示多个基因编码直接催化产生FVBP化合物的生化反应或在形成FVBP化合物之前参与代谢物通量的蛋白质。据推测，多种转录因子参与了所有必要基因的精确调控，导致MD花的特定挥发性特征。在获得与拟南芥R2 R3-MYB转录因子具有同源性的所有可用矮牵牛转录物序列后，鉴定、克隆和表征PhMYB 4（因其与AtMYB 4的接近同一性而命名）。PhMYB 4转录积累到相对较高的水平，在开花期和整个开放的花的组织，这与FVBP生产和排放的空间和发育分布相一致。在RNAi抑制PhMYB 4（ir-PhMYB 4）后，矮牵牛肉桂酸-4-羟化酶（PhC 4 H1和PhC 4 H2）基因转录物水平均显著增加。此外，与MD相比，ir-PhMYB 4植物释放出更高水平的源自对香豆酸（异丁香酚和丁香酚）的FVBP化合物。总之，这些结果表明PhMYB 4在抑制C4 H转录中起作用，间接控制矮牵牛花组织中FVBP产生的平衡（即微调）。
In Petunia×hybrida cv ‘Mitchell Diploid’ (MD), floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is controlled spatially, developmentally, and daily at molecular, metabolic, and biochemical levels. Multiple genes have been shown to encode proteins that either directly catalyse a biochemical reaction yielding FVBP compounds or are involved in metabolite flux prior to the formation of FVBP compounds. It was hypothesized that multiple transcription factors are involved in the precise regulation of all necessary genes, resulting in the specific volatile signature of MD flowers. After acquiring all available petunia transcript sequences with homology to Arabidopsis thaliana R2R3-MYB transcription factors, PhMYB4 (named for its close identity to AtMYB4) was identified, cloned, and characterized. PhMYB4 transcripts accumulate to relatively high levels in floral tissues at anthesis and throughout open flower stages, which coincides with the spatial and developmental distribution of FVBP production and emission. Upon RNAi suppression of PhMYB4 (ir-PhMYB4) both petunia CINNAMATE-4-HYDROXYLASE (PhC4H1 and PhC4H2) gene transcript levels were significantly increased. In addition, ir-PhMYB4 plants emit higher levels of FVBP compounds derived from p-coumaric acid (isoeugenol and eugenol) compared with MD. Together, these results indicate that PhMYB4 functions in the repression of C4H transcription, indirectly controlling the balance of FVBP production in petunia floral tissue (i.e. fine-tunes).
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影响因子：
5.1
作者：
Jorgensen, RA;Cluster, PD;Napoli, CA
通讯作者：
Napoli, CA
影响因子：
7.4
作者：
Underwood, BA;Tieman, DM;Clark, DG
通讯作者：
Clark, DG
影响因子：
11.6
作者：
Maeda, Hiroshi;Shasany, Ajit K.;Dudareva, Natalia
通讯作者：
Dudareva, Natalia
DOI：
10.1073/pnas.0603732103
发表时间：
2006-06-27
影响因子：
11.1
作者：
Koeduka, Takao;Fridman, Eyal;Pichersky, Eran
通讯作者：
Pichersky, Eran
影响因子：
11.6
作者：
Pan, Jianwei;Fujioka, Shozo;Chen, Rujin
通讯作者：
Chen, Rujin