Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation.
Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation.
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DOI：
10.1186/s13041-014-0060-3
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发表时间：
2014-08-19
期刊：
影响因子：
3.6
通讯作者：
Collingridge GL
中科院分区：
文献类型：
--
作者：
Ng AN;Doherty AJ;Lombroso PJ;Emptage NJ;Collingridge GL
内质网（ER）在树突棘内是能动的，但其调控机制尚不清楚。为了解决这个问题，我们同时成像形态和ER含量的树突棘培养分离的小鼠海马神经元。在10分钟的时间内，棘是高度动态的，棘的体积增加和减少。ER存在于大约50%的脊柱中，并且也是高度动态的，在这段时间内净增加。抑制NMDA受体的内源性激活导致ER生长减少。相反，通过消除纹状体富集的蛋白酪氨酸磷酸酶（STEP）来增强NMDA受体的突触激活，导致增强的ER生长。因此，NMDA受体迅速调节脊柱ER动力学。
Endoplasmic reticulum (ER) is motile within dendritic spines, but the mechanisms underlying its regulation are poorly understood. To address this issue, we have simultaneously imaged morphology and ER content of dendritic spines in cultured dissociated mouse hippocampal neurons. Over a 10 min period, spines were highly dynamic, with spines both increasing and decreasing in volume. ER was present in approximately 50% of spines and was also highly dynamic, with a net increase over this period of time. Inhibition of the endogenous activation of NMDA receptors resulted in a reduction in ER growth. Conversely, augmentation of the synaptic activation of NMDA receptors, by elimination of striatal-enriched protein tyrosine phosphatase (STEP), resulted in enhanced ER growth. Therefore, NMDA receptors rapidly regulate spine ER dynamics.
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