Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing.
Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing.
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DOI：
10.1038/s41598-023-43120-y
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发表时间：
2023-10-18
影响因子：
4.6
通讯作者：
Cheng, Zixi
中科院分区：
文献类型：
--
作者：
Ma, Jichao;Duyen Nguyen;Madas, Jazune;Kwiat, Andrew M.;Toledo, Zulema;Bizanti, Ariege;Kogut, Nicole;Mistareehi, Anas;Bendowski, Kohlton;Zhang, Yuanyuan;Chen, Jin;Li, De-Pei;Powley, Terry L.;Furness, John B.;Cheng, Zixi
背根神经节（DRG）将脊髓传入轴突投射至胃部。然而，脊髓传入轴突在胃内的分布和形态尚未得到充分描述。在本研究中，我们综合运用了一系列先进技术，包括向左侧T7 - T11背根神经节注射顺行示踪剂、采用抗生物素蛋白 - 生物素及铜酸蓝标记法、使用蔡司M2成像仪以及Neurolucida软件，对大鼠全胃肌壁平铺标本中的脊髓传入轴突进行特征分析。我们发现，脊髓传入轴突支配胃的所有区域，且具有多种不同的终末结构，支配着不同的胃部靶点：（1）神经节型：部分轴突与肌间神经节内的单个神经元形成串珠样接触。（2）肌肉型：大多数轴突与纵行肌和环行肌平行走行，并呈现球状膨大。在环行肌层内观察到复杂的终末结构。（3）神经节 - 肌肉混合型：部分单个串珠样轴突同时支配肌间神经元和环行肌，呈现出多形性终末结构。（4）血管型：单个串珠样轴突沿血管走行，偶尔穿过血管壁。这项工作为今后在正常及病理生理条件下，对脊髓传入轴突支配胃的局部解剖学和功能图谱研究奠定了基础。
The dorsal root ganglia (DRG) project spinal afferent axons to the stomach. However, the distribution and morphology of spinal afferent axons in the stomach have not been well characterized. In this study, we used a combination of state-of-the-art techniques, including anterograde tracer injection into the left DRG T7-T11, avidin–biotin and Cuprolinic Blue labeling, Zeiss M2 Imager, and Neurolucida to characterize spinal afferent axons in flat-mounts of the whole rat stomach muscular wall. We found that spinal afferent axons innervated all regions with a variety of distinct terminal structures innervating different gastric targets: (1) The ganglionic type: some axons formed varicose contacts with individual neurons within myenteric ganglia. (2) The muscle type: most axons ran in parallel with the longitudinal and circular muscles and expressed spherical varicosities. Complex terminal structures were observed within the circular muscle layer. (3) The ganglia-muscle mixed type: some individual varicose axons innervated both myenteric neurons and the circular muscle, exhibiting polymorphic terminal structures. (4) The vascular type: individual varicose axons ran along the blood vessels and occasionally traversed the vessel wall. This work provides a foundation for future topographical anatomical and functional mapping of spinal afferent axon innervation of the stomach under normal and pathophysiological conditions.
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影响因子：
2.5
作者：
Gomez-Alvarez, Marcelo;Saldana, Enrique
通讯作者：
Saldana, Enrique
DOI：
10.1152/ajpgi.00019.2022
发表时间：
2022-05-01
影响因子：
4.5
作者：
Dodds, Kelsi N.;Travis, Lee;Spencer, Nick J.
通讯作者：
Spencer, Nick J.
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3
作者：
Numazaki, Mitsuko;Tominaga, Makoto
通讯作者：
Tominaga, Makoto
影响因子：
3.3
作者：
FURNESS, JB;PAPKA, RE;ESKAY, RL
通讯作者：
ESKAY, RL
影响因子：
3.3
作者：
Cameron, OG
通讯作者：
Cameron, OG