A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications.
A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications.
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DOI：
10.1039/d1an01548f
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发表时间：
2022-04-11
期刊：
影响因子：
--
通讯作者：
Stone N
中科院分区：
文献类型：
--
作者：
Gosling S;Calabrese D;Nallala J;Greenwood C;Pinder S;King L;Marks J;Pinto D;Lynch T;Lyburn ID;Hwang ES;Grand Challenge Precision Consortium;Rogers K;Stone N
导管原位癌（DCIS）常与乳腺钙化有关。这项研究结合了多种分析技术，在微米尺度上调查这些钙化的异质性。X射线衍射、扫描电子显微镜、拉曼和傅里叶变换红外光谱被用来确定位于福尔马林固定石蜡包埋DCIS乳腺组织样本中的II型乳腺钙化的物理化学和晶体学性质。在钙化中识别出多个磷酸钙相，以不同的模式分布。羟基磷灰石是主要矿物，在钙化边缘发现镁白磷钙石。无定形磷酸钙和磷酸八钙也被确定为接近钙化边缘的明显矿物/基质屏障。羟基磷灰石的晶体学特征在钙化处也各不相同，中心结晶度较高，钙化边缘碳酸盐取代最高。蛋白质在钙化和周围软组织中的分布也有差异，胶原蛋白和β-折叠蛋白特征存在不同程度。本研究中分析技术的结合对于了解乳腺钙化的异质性以及如何将钙化的晶体学和物理化学性质与周围组织微环境联系起来至关重要。结合晶体学和光谱学的方法被用来调查乳腺钙化的异质性发现与导管原位癌，揭示了独特的蛋白质分布和矿物成分的模式。
Ductal carcinoma in situ (DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform infrared spectroscopy were used to determine the physicochemical and crystallographic properties of type II breast calcifications located in formalin fixed paraffin embedded DCIS breast tissue samples. Multiple calcium phosphate phases were identified across the calcifications, distributed in different patterns. Hydroxyapatite was the dominant mineral, with magnesium whitlockite found at the calcification edge. Amorphous calcium phosphate and octacalcium phosphate were also identified close to the calcification edge at the apparent mineral/matrix barrier. Crystallographic features of hydroxyapatite also varied across the calcifications, with higher crystallinity centrally, and highest carbonate substitution at the calcification edge. Protein was also differentially distributed across the calcification and the surrounding soft tissue, with collagen and β-pleated protein features present to differing extents. Combination of analytical techniques in this study was essential to understand the heterogeneity of breast calcifications and how this may link crystallographic and physicochemical properties of calcifications to the surrounding tissue microenvironment. Combined crystallographic and spectroscopic methods were used to investigate the heterogeneity of breast calcifications found associated with ductal carcinoma in situ, revealing distinctive patterns in protein distribution and mineral composition.
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影响因子：
14
作者：
Fleet, Michael E.;Liu, Xi
通讯作者：
Liu, Xi
影响因子：
3.3
作者：
FRAPPART, L;BOUDEULLE, M;FEROLDI, J
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14
作者：
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影响因子：
5.5
作者：
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通讯作者：
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影响因子：
1.8
作者：
MONTEL, G;BONEL, G;REY, C
通讯作者：
REY, C