Multiple monoaminergic modulation of posturo-locomotor network activity in the newborn rat spinal cord.
Multiple monoaminergic modulation of posturo-locomotor network activity in the newborn rat spinal cord.
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DOI：
10.3389/fncir.2014.00099
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发表时间：
2014
影响因子：
3.5
通讯作者：
Cazalets JR
中科院分区：
文献类型：
--
作者：
Beliez L;Barrière G;Bertrand SS;Cazalets JR
致力于了解运动控制的研究主要涉及控制腿部运动的神经回路的功能，而对激活躯干肌肉与肢体运动协调的网络的运作知之甚少。本工作的目的是：（1）确定最强烈激活姿势性胸回路的外源性神经递质鸡尾酒;（2）研究生物胺5-羟色胺（5-HT）、多巴胺（DA）和去甲肾上腺素（NA）如何调节肢体和轴向运动网络之间的协调。实验在离体新生大鼠脊髓标本上进行。我们从腹根进行记录以监测后肢运动和轴向姿势网络活动。三种胺与兴奋性氨基酸（EAAs）的每种组合在具有特定特征的所有节段水平引起协调的节律性运动活动。5-HT和DA的周期变异性相似，而NA的周期变异性显着较高。与5-HT和NA相比，DA在胸段引起幅度较小的运动爆发，而DA和NA在腰段和骶段引起幅度高于5-HT的运动爆发。胺调制的相位关系的爆发在各个节段相对于参考腰椎节段。在胸部水平有一个相位滞后之间的所有记录段在5-HT的存在下，而DA和NA引起同步爆发。在骶骨水平，5-HT和DA引起的节段间相移，而与NA的关系成为锁相。EAA与两种或甚至所有三种胺的各种组合引起了有节奏的运动输出，这比单独使用一种胺更可变。我们的研究结果提供了新的数据脊髓网络之间的协调过程，表明每一种胺有一个特征性的“签名”，其具体影响intersegmentate相位关系。
Studies devoted to understanding locomotor control have mainly addressed the functioning of the neural circuits controlling leg movements and relatively little is known of the operation of networks that activate trunk muscles in coordination with limb movements. The aim of the present work was (1) to identify the exogenous neurotransmitter cocktail that most strongly activates postural thoracic circuitry; (2) to investigate how the biogenic amines serotonin (5-HT), dopamine (DA), and noradrenaline (NA) modulate the coordination between limb and axial motor networks. Experiments were carried out on in vitro isolated spinal cord preparations from newborn rats. We recorded from ventral roots to monitor hindlimb locomotor and axial postural network activity. Each combination of the three amines with excitatory amino acids (EAAs) elicited coordinated rhythmic motor activity at all segmental levels with specific characteristics. The variability in cycle period was similar with 5-HT and DA while it was significantly higher with NA. DA elicited motor bursts of smaller amplitude in thoracic segments compared to 5-HT and NA, while both DA and NA elicited motor bursts of higher amplitude than 5-HT in the lumbar and sacral segments. The amines modulated the phase relationships of bursts in various segments with respect to the reference lumbar segment. At the thoracic level there was a phase lag between all recorded segments in the presence of 5-HT, while DA and NA elicited synchronous bursting. At the sacral level, 5-HT and DA induced an intersegmental phase shift while relationships became phase-locked with NA. Various combinations of EAAs with two or even all three amines elicited rhythmic motor output that was more variable than with one amine alone. Our results provide new data on the coordinating processes between spinal cord networks, demonstrating that each amine has a characteristic “signature” regarding its specific effect on intersegmental phase relationships.
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影响因子：
5.5
作者：
Falgairolle, Melanie;Cazalets, Jean-Rene
通讯作者：
Cazalets, Jean-Rene
影响因子：
2.5
作者：
Gabbay, H;Delvolvé, I;Lev-Tov, A
通讯作者：
Lev-Tov, A
DOI：
10.1098/rspb.1998.0542
发表时间：
1998-11-07
影响因子：
4.7
作者：
Beato, M;Nistri, A
通讯作者：
Nistri, A
影响因子：
2.5
作者：
DUENAS, SH;LOEB, GE;MARKS, WB
通讯作者：
MARKS, WB
影响因子：
3.4
作者：
Faumont, S;Combes, D;Simmers, J
通讯作者：
Simmers, J