Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
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新型隐球菌中 miRNA 的鉴定和功能验证
DOI：
10.1371/journal.pone.0052734
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Jiang N;Yang Y;Janbon G;Pan J;Zhu X
微小RNA（miRNA）是植物和动物中通过同源mRNA碱基配对而产生的内源性转录后抑制因子，人们大多认为它在真菌王国中消失了。在这里，我们报告了从真菌新型隐球菌中鉴定出的miRNA。利用生物信息学和北方印迹技术，我们发现这些miRNAs及其发夹前体在该真菌中存在。miR 1和miR 2的大小分别为22 nt和18 nt。前体的长度约为1070 nt，接近哺乳动物pre-miRNA。miR 1/2中也发现了miRNAs的特征。我们证明了所鉴定的miRNAs，miR 1和miR 2，通过典型的RNAi途径引起转基因沉默。生物信息学分析揭示了miR 1/2在转座因子（transposable elements，TEs）和假基因中的大量相同序列，提示真菌miRNAs可能参与调节转座子的活性和假基因的表达。本研究在C. neoformans，揭示了真核miRNA的多样性和进化起源。
microRNAs (miRNAs), endogenous posttranscriptional repressors by base-pairing of their cognate mRNAs in plants and animals, have mostly been thought lost in the kingdom of fungi. Here, we report the identification of miRNAs from the fungus Cryptococcus neoformans. With bioinformatics and Northern blotting approaches, we found that these miRNAs and their hairpin precursors were present in this fungus. The size of miR1 and miR2 is 22 nt and 18 nt, respectively. The precursors are about ∼70 nt in length that is close to mammalian pre-miRNAs. Characteristic features of miRNAs are also found in miR1/2. We demonstrated that the identified miRNAs, miR1 and miR2, caused transgene silencing via the canonical RNAi pathway. Bioinformantics analysis helps to reveal a number of identical sequences of the miR1/2 in transposable elements (TEs) and pseudogenes, prompting us to think that fungal miRNAs might be involved in the regulation of the activity of transposons and the expression of pseudogenes. This study identified functional miRNAs in C. neoformans, and sheds light on the diversity and evolutionary origin of eukaryotic miRNAs.
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影响因子：
3
作者：
Janbon, Guilhem;Maeng, Shinae;Yang, Dong-Hoon;Ko, Young-Joon;Jung, Kwang-Woo;Moyrand, Frederique;Floyd, Anna;Heitman, Joseph;Bahn, Yong-Sun
通讯作者：
Bahn, Yong-Sun
影响因子：
64.5
作者：
Halic M;Moazed D
通讯作者：
Moazed D
影响因子：
4.4
作者：
Jiang, Nan;Xiao, Dongguang;Zhu, Xudong
通讯作者：
Zhu, Xudong
影响因子：
64.8
作者：
Denli, AM;Tops, BBJ;Hannon, GJ
通讯作者：
Hannon, GJ
影响因子：
64.5
作者：
Grishok, A;Pasquinelli, AE;Mello, CC
通讯作者：
Mello, CC