Characterizing the role of RNA silencing components in Cryptococcus neoformans.
Characterizing the role of RNA silencing components in Cryptococcus neoformans.
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DOI：
10.1016/j.fgb.2010.10.005
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发表时间：
2010-12
影响因子：
3
通讯作者：
Bahn, Yong-Sun
中科院分区：
文献类型：
--
作者：
Janbon, Guilhem;Maeng, Shinae;Yang, Dong-Hoon;Ko, Young-Joon;Jung, Kwang-Woo;Moyrand, Frederique;Floyd, Anna;Heitman, Joseph;Bahn, Yong-Sun
RNA干扰（RNA interference, RNAi）通过小RNA分子同源性降解靶mRNA介导，在控制许多真核生物的转录和翻译过程中起着关键作用。RNAi机制在包括病原真菌在内的多种真菌物种中也具有进化保守性。为了阐明RNAi途径在引起真菌性脑膜炎的新型隐球菌中的生理功能，我们对血清型A和dc新生隐球菌中Argonaute （AGO1和AGO2）、RNA依赖性RNA聚合酶（RDP1）和Dicers （DCR1和DCR2）的编码基因进行了遗传分析。本研究表明，Ago1、Rdp1和Dcr2是新生C.发生的RNAi过程的主要组成部分。然而，RNAi机制并不参与两种毒力因子（荚膜和黑色素）的产生、性别分化和各种应激反应的调节。对血清型A和D RNAi突变体的比较转录组分析显示，当RNAi过程受到干扰时，全基因组转录组谱仅发生轻微变化。值得注意的是，血清型D rdp1Δ突变体显示出活性反转录转座子和转座子的转录丰度增加，如T2和T3，后者是新生C.的新型血清型D特异性转座子。在野生型背景下，T2和T3都被发现是弱活性的移动元件，尽管我们没有发现Cnl1反转录转座子移动的证据。相反，所有三个转座元件在rdp1Δ突变株中表现出增强的移动性。综上所述，RNAi途径在控制新生C.转座子活性和基因组完整性方面发挥了重要作用。
The RNA interference (RNAi) mediated by homology-dependent degradation of the target mRNA with small RNA molecules plays a key role in controlling transcription and translation processes in a number of eukaryotic organisms. The RNAi machinery is also evolutionarily conserved in a wide variety of fungal species, including pathogenic fungi. To elucidate the physiological functions of the RNAi pathway in Cryptococcus neoformans that causes fungal meningitis, here we performed genetic analyses for genes encoding Argonaute (AGO1 and AGO2), RNA-dependent RNA polymerase (RDP1), and Dicers (DCR1 and DCR2) in both serotype A and D C. neoformans. The present study shows that Ago1, Rdp1, and Dcr2 are the major components of the RNAi process occurring in C. neoformans. However, the RNAi machinery is not involved in regulation of production of two virulence factors (capsule and melanin), sexual differentiation, and diverse stress response. Comparative transcriptome analysis of the serotype A and D RNAi mutants revealed that only modest changes occur in the genome-wide transcriptome profiles when the RNAi process was perturbed. Notably, the serotype D rdp1Δ mutants showed an increase in transcript abundance of active retrotransposons and transposons, such as T2 and T3, the latter of which is a novel serotype D-specific transposon of C. neoformans. In a wild type background both T2 and T3 were found to be weakly active mobile elements, although we found no evidence of Cnl1 retrotransposon mobility. In contrast, all three transposable elements exhibited enhanced mobility in the rdp1Δ mutant strain. In conclusion, the RNAi pathway plays an important role in controlling transposon activity and genome integrity of C. neoformans.
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影响因子：
3.3
作者：
Bahn, YS;Kojima, K;Heitman, J
通讯作者：
Heitman, J
影响因子：
2.6
作者：
Goodwin, TJD;Poulter, RTM
通讯作者：
Poulter, RTM
影响因子：
3.3
作者：
Kim, Min Su;Ko, Young-Joon;Bahn, Yong-Sun
通讯作者：
Bahn, Yong-Sun
影响因子：
--
作者：
Hicks, JK;D'Souza, CA;Heitman, J
通讯作者：
Heitman, J
影响因子：
3.4
作者：
DeMarco R;Venancio TM;Verjovski-Almeida S
通讯作者：
Verjovski-Almeida S