Lymphoid and other tissue-specific phenotypes of polyomavirus enhancer recombinants: positive and negative combinational effects on enhancer specificity and activity
Lymphoid and other tissue-specific phenotypes of polyomavirus enhancer recombinants: positive and negative combinational effects on enhancer specificity and activity
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多瘤病毒增强子重组体的淋巴和其他组织特异性表型：对增强子特异性和活性的正向和负向组合效应
DOI：
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发表时间：
1986
影响因子：
5.3
通讯作者：
L. Villarreal
中科院分区：
文献类型：
--
作者：
B. A. Campbell;L. Villarreal
构建了多瘤病毒（Py）非编码区的异源增强子重组体和缺失，并分析了许多淋巴细胞和其他细胞系中DNA复制和转录的组织特异性。将猿猴病毒40个72碱基对重复序列、小鼠免疫球蛋白重链增强子和莫洛尼鼠白血病病毒增强子插入Py的PvuII-D位点（核苷酸5128至5265）。测定了这些重组体和亲本PvuII-D缺失突变体在允许的3 T6细胞和MOP-6细胞以及非允许的小鼠B淋巴细胞、T淋巴细胞、肥大细胞和胚胎癌细胞中复制的能力。野生型Py DNA不允许在大多数淋巴细胞系中复制，除了一个杂交瘤细胞系。然而，简单地删除Py PvuII-D区域，使Py的宿主范围扩大，允许在一些T淋巴细胞和肥大细胞瘤细胞系中高水平复制，表明该元件可以是组织特异性阴性和阳性元件。用鼠白血病病毒增强子取代Py PvuII-D产生了Py基因组，该基因组保留了在3 T6细胞中复制的能力，但在B淋巴细胞中也复制良好。用免疫球蛋白重链增强子取代允许在B淋巴细胞中复制，但干扰3 T6细胞和肥大细胞瘤中的复制。令人惊讶的是，用猿猴病毒40个72碱基对增强子重复序列取代得到的重组体不会在任何尝试的细胞系（包括MOP-6细胞）中复制，即使具有该增强子的其他重组体会复制。因此，我们观察到增强子组分和Py基因组之间的这些组合中的合作和干扰，并且这些组合的活性是细胞特异性的。这些结果作为证据，可能有一个位置依赖，或语法，识别控制Py组织特异性的遗传因素。
Heterologous enhancer recombinants and deletions of the polyomavirus (Py) noncoding region were constructed and analyzed for tissue specificity of DNA replication and transcription in a number of lymphoid and other cell lines. The simian virus 40 72-base-pair repeat, mouse immunoglobulin heavy-chain enhancer, and Moloney murine leukemia virus enhancer were inserted into the PvuII-D locus (nucleotides 5128 through 5265) of Py. The ability of these recombinants and the parental PvuII-D deletion mutant to replicate in permissive 3T6 cells and MOP-6 cells as well as in nonpermissive mouse B lymphoid, T lymphoid, mastocyte, and embryonal carcinoma cells was determined. Wild-type Py DNA was not permissive for replication in most lymphoid cell lines, except one hybridoma line. Simply deleting the Py PvuII-D region, however, gave Py an expanded host range, allowing high-level replication in some T lymphoid and mastocytoma cell lines, indicating that this element can be a tissue-specific negative as well as positive element. Substitution of the murine leukemia virus enhancer for Py PvuII-D yielded a Py genome which retained the ability to replicate in 3T6 cells but also replicated well in B lymphoid cells. Substitution with the immunoglobulin heavy-chain enhancer allowed replication in B lymphoid cells but interfered with replication in 3T6 cells and mastocytomas. Surprisingly, substitution with the simian virus 40 72-base-pair enhancer repeat gave a recombinant which would not replicate in any cell line tried, including MOP-6 cells, even though other recombinants with this enhancer would replicate. Thus, we observed both cooperation and interference in these combinations between enhancer components and the Py genome and that these combined activities were cell specific. These results are presented as evidence that there may be a positional dependence, or syntax, for the recognition of genetic elements controlling Py tissue specificity.
一种分泌 IL 2 的 T 细胞杂交瘤，可对 H-2D 区域的自身 I 类组织相容性抗原做出反应。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Endres,RO;Marrack,P;Kappler,JW
通讯作者：
Kappler,JW
通过体内重组和选择从噬菌体文库中纯化基因组序列。
影响因子：
14.9
作者：
Seed,B
通讯作者：
Seed,B
有效感染 F9 胚胎癌细胞的多瘤突变体不能拯救 F9 细胞中的野生型多瘤。
DOI：
10.1073/pnas.79.5.1479
发表时间：
1982
影响因子：
11.1
作者：
Fujimura,FK;Linney,E
通讯作者：
Linney,E