Transcript profiling reveals mechanisms for lipid conservation during diapause in the mosquito, Aedes albopictus.
Transcript profiling reveals mechanisms for lipid conservation during diapause in the mosquito, Aedes albopictus.
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DOI：
10.1016/j.jinsphys.2012.04.013
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发表时间：
2012-07
影响因子：
2.2
通讯作者：
Denlinger, David L.
中科院分区：
文献类型：
--
作者：
Reynolds, Julie A.;Poelchau, Monica F.;Rahman, Zahra;Armbruster, Peter A.;Denlinger, David L.
关键词：
亚洲虎蚊（白纹伊蚊）是一种医学上重要的入侵物种，其地理分布在过去 20 年中急剧扩大，其成功的关键因素之一是其作为滞育的甲虫一龄幼虫（包裹在卵绒毛膜内）能够在长途运输中存活下来。我们报告说，进入滞育的噬菌体幼虫比非滞育幼虫体型更大，脂质含量也高出 30%。为了加深我们对滞育期间脂质代谢分子调控的理解，我们使用 qRT-PCR 评估了 21 个基因的相对 mRNA 丰度。胚胎发育过程中脂质储存滴蛋白 2 的表达升高可能导致我们在滞育个体中发现更高的脂质含量。滞育期间脂质的保守反映在参与脂质分解代谢的基因的下调，包括脂肪酶 2、脂肪酶 3、脂肪酶 4、酰基辅酶 A 脱氢酶 4 和异戊酰辅酶 A 脱氢酶。参与脂肪酸合成和修饰的两个基因，Δ(9)-去饱和酶和脂肪酰辅酶A延伸酶，在滞育的噬菌体幼虫中均上调，表明它们的基因产物分别在产生不饱和脂肪酸以增强低温下膜流动性和产生抵抗干燥所需的表面碳氢化合物的前体方面发挥作用。总之，这些结果表明滞育程序导致胚胎内的脂质代谢存在显着差异，并且这些差异既发生在滞育实际开始之前，也发生在滞育状态期间。
The Asian tiger mosquito, Aedes albopictus, is a medically important invasive species whose geographic distribution has expanded dramatically during the past 20 years, and one of the key elements of its success is its capacity to survive long distance transport as a diapausing pharate first instar larva, encased within the chorion of the egg. We report that pharate larvae entering diapause are larger and contain 30% more lipid than their nondiapausing counterparts. To improve our understanding of the molecular regulation of lipid metabolism during diapause, we assessed the relative mRNA abundance of 21 genes using qRT-PCR. Elevated expression of lipid storage droplet protein 2 during embryonic development likely contributes to the higher amounts of lipid we noted in diapausing individuals. The conservation of lipids during diapause is reflected in downregulation of genes involved in lipid catabolism, including lipase 2, lipase 3, lipase 4, acyl-CoA dehydrogenase 4, and isovaleryl-CoA dehydrogenase. Two genes involved in fatty acid synthesis and modification, Δ(9)-desaturase, and fatty acyl-CoA elongase, were both upregulated in diapausing pharate larvae, suggesting roles for their gene products in generating unsaturated fatty acids to enhance membrane fluidity at low temperatures and generating precursors to the surface hydrocarbons needed to resist desiccation, respectively. Together, the results point to substantial distinctions in lipid metabolism within the embryo as a consequence of the diapause program, and these differences occur both before the actual onset of diapause as well as during the diapause state.
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影响因子：
4.4
作者：
Castro C;Sar F;Shaw WR;Mishima M;Miska EA;Griffin JL
通讯作者：
Griffin JL
DOI：
10.1590/s0074-02762009000100020
发表时间：
2009-02-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Farnesi, Luana Cristina;Martins, Ademir Jesus;Rezende, Gustavo Lazzaro
通讯作者：
Rezende, Gustavo Lazzaro
影响因子：
29
作者：
Hulver, MW;Berggren, JR;Muoio, DM
通讯作者：
Muoio, DM
影响因子：
2.1
作者：
Benedict, Mark Q.;Levine, Rebecca S.;Lounibos, L. Philip
通讯作者：
Lounibos, L. Philip
影响因子：
2.2
作者：
BLOMQUIST, GJ;NELSON, DR;DERENOBALES, M
通讯作者：
DERENOBALES, M