Moderate DNA methylation changes associated with nitrogen remobilization and leaf senescence in Arabidopsis.
Moderate DNA methylation changes associated with nitrogen remobilization and leaf senescence in Arabidopsis.
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DOI：
10.1093/jxb/erac167
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发表时间：
2022-08-11
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
--
作者：
DNA甲基化缺陷的拟南芥突变体改变了衰老，叶片甲基化模式在衰老过程中适度变化，但与衰老相关基因关系不大。植物的寿命受到环境和遗传因素的限制。在向生殖生长过渡后，叶子衰老结束了单果植物的细胞生命，将养分重新转移到储存器官。在拟南芥中，我们最初观察到两个甲基化突变体（ros1 和三重 dmr1/2 cmt3 敲除）中叶片与种子比例的改变、更快的衰老进程、瞬时脱氮后叶片氮回收的改变以及最终增强的叶片氮再动员。对野生型 Col-0 叶子中 DNA 甲基化组的分析发现，胞嘧啶甲基化随着叶子的逐渐衰老而出现最初的适度下降，主要是在 CG 背景下。晚衰老与胞嘧啶的中度从头甲基化相关，主要是在 CHH 背景下。存在相对较少的差异甲基化区域，包括 ROS1 启动子中与 ROS1 下调相关的一个区域，但这些区域与已知的衰老相关基因无关。在转录因子结合位点（例如 WRKY 靶向的 W 盒）中发现了差异甲基化模式。人工结合位点的甲基化损害了体外转录因子的结合。然而，目前尚不清楚叶片衰老过程中适度的甲基化组变化如何与衰老过程中上调的基因相关。
Arabidopsis mutants defective in DNA methylation have altered senescence, and leaf methylation patterns moderately change during ageing, but there is little relation to senescence-associated genes. The lifespan of plants is restricted by environmental and genetic components. Following the transition to reproductive growth, leaf senescence ends cellular life in monocarpic plants to remobilize nutrients to storage organs. In Arabidopsis, we initially observed altered leaf to seed ratios, faster senescence progression, altered leaf nitrogen recovery after transient nitrogen removal, and ultimately enhanced nitrogen remobilization from the leaves in two methylation mutants (ros1 and the triple dmr1/2 cmt3 knockout). Analysis of the DNA methylome in wild type Col-0 leaves identified an initial moderate decline of cytosine methylation with progressing leaf senescence, predominantly in the CG context. Late senescence was associated with moderate de novo methylation of cytosines, primarily in the CHH context. Relatively few differentially methylated regions, including one in the ROS1 promoter linked to down-regulation of ROS1, were present, but these were unrelated to known senescence-associated genes. Differential methylation patterns were identified in transcription factor binding sites, such as the W-boxes that are targeted by WRKYs. Methylation in artificial binding sites impaired transcription factor binding in vitro. However, it remains unclear how moderate methylome changes during leaf senescence are linked with up-regulated genes during senescence.
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影响因子：
7.7
作者：
Halter T;Wang J;Amesefe D;Lastrucci E;Charvin M;Singla Rastogi M;Navarro L
通讯作者：
Navarro L
影响因子：
5.6
作者：
Doll, Jasmin;Muth, Maren;Zentgraf, Ulrike
通讯作者：
Zentgraf, Ulrike
影响因子：
7.4
作者：
Diaz, Celine;Lemaitre, Thomas;Masclaux-Daubresse, Celine
通讯作者：
Masclaux-Daubresse, Celine
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.
影响因子：
7.2
作者：
Ay, Nicole;Irmler, Kristina;Humbeck, Klaus
通讯作者：
Humbeck, Klaus