Magnetic resonance imaging provides evidence of glymphatic drainage from human brain to cervical lymph nodes.
Magnetic resonance imaging provides evidence of glymphatic drainage from human brain to cervical lymph nodes.
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DOI：
10.1038/s41598-018-25666-4
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发表时间：
2018-05-08
影响因子：
4.6
通讯作者：
Ringstad G
中科院分区：
文献类型：
--
作者：
Eide PK;Vatnehol SAS;Emblem KE;Ringstad G
啮齿动物的临床前研究提供了中枢神经系统（CNS）具有功能性淋巴管的证据。然而，在人体内的观察并没有得到证实。我们在此展示了用鞘内造影剂作为脑脊液（CSF）示踪剂增强的磁共振成像（MRI）在体内对CNS淋巴引流到颈部淋巴结的数据。19例患者在鞘内注射造影剂前和注射后24-48小时内进行了颅内室和颈部的标准化MRI检查。造影增强通过额下回附近脑脊液、额下回脑实质、海马旁回、丘脑、脑桥及颈部淋巴结实质的信号改变证实，矢状窦和颈部肌肉分别作为颅脑和颈部MRI获取的参比组织。信号强度变化的时间序列显示脑脊液内造影剂增强先于淋巴增强，并在鞘内注射后4-6小时达到峰值。颈部淋巴结增强与淋巴增强高峰时间重合，在24小时后达到高峰。我们的研究结果提供了脑脊液示踪剂引流到人类颈部淋巴结的体内证据。淋巴结增强时间与脑淋巴增强时间一致，与脑脊液增强时间不一致。
Pre-clinical research in rodents provides evidence that the central nervous system (CNS) has functional lymphatic vessels. In-vivo observations in humans, however, are not demonstrated. We here show data on CNS lymphatic drainage to cervical lymph nodes in-vivo by magnetic resonance imaging (MRI) enhanced with an intrathecal contrast agent as a cerebrospinal fluid (CSF) tracer. Standardized MRI of the intracranial compartment and the neck were acquired before and up to 24–48 hours following intrathecal contrast agent administration in 19 individuals. Contrast enhancement was radiologically confirmed by signal changes in CSF nearby inferior frontal gyrus, brain parenchyma of inferior frontal gyrus, parahippocampal gyrus, thalamus and pons, and parenchyma of cervical lymph node, and with sagittal sinus and neck muscle serving as reference tissue for cranial and neck MRI acquisitions, respectively. Time series of changes in signal intensity shows that contrast enhancement within CSF precedes glymphatic enhancement and peaks at 4–6 hours following intrathecal injection. Cervical lymph node enhancement coincides in time with peak glymphatic enhancement, with peak after 24 hours. Our findings provide in-vivo evidence of CSF tracer drainage to cervical lymph nodes in humans. The time course of lymph node enhancement coincided with brain glymphatic enhancement rather than with CSF enhancement.
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影响因子：
1.4
作者：
Lüdemann, W;von Rautenfeld, DB;Brinker, T
通讯作者：
Brinker, T
影响因子：
12.7
作者：
PETTERSSON, CAV
通讯作者：
PETTERSSON, CAV
影响因子：
7.7
作者：
Absinta M;Ha SK;Nair G;Sati P;Luciano NJ;Palisoc M;Louveau A;Zaghloul KA;Pittaluga S;Kipnis J;Reich DS
通讯作者：
Reich DS
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M
DOI：
10.1016/j.jacr.2015.11.009
发表时间：
2016-03
期刊：
Journal of the American College of Radiology : JACR
影响因子：
--
作者：
Malayeri AA;Brooks KM;Bryant LH;Evers R;Kumar P;Reich DS;Bluemke DA
通讯作者：
Bluemke DA