The N-glycome of human plasma.
The N-glycome of human plasma.
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DOI：
10.1021/pr100528k
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发表时间：
2010-09-03
影响因子：
4.4
通讯作者：
Reinhold, Vernon N.
中科院分区：
文献类型：
--
作者：
Stumpo, Katherine A.;Reinhold, Vernon N.
已使用离子阱仪器（ITMSn）通过质谱法对从人血浆蛋白中分离的N-连接聚糖进行了分析和测序。将释放的聚糖制备为还原的甲基化类似物，并直接注入基于芯片的纳米电喷雾电离系统中，并通过ITMSn进行分析。对比IgG消除和非消除血浆样本的质量曲线（MS1），并再次将这些结果与最近的文献报告进行比较。在耗尽之前，检测到大约50个独立的聚糖离子，耗尽之后这增加了一倍多，达到106个。质量范围为1K-5KDa，其中包括许多双电荷和三电荷离子，这些离子可通过更高的MS分辨率分辨。将耗尽样品中的选定离子分解以限定其详细结构，从而提供高性能测序结果。这种非色谱、直接输注和气相结构表征的简单性与使用替代仪器和辅助技术的最新报告相比最为有利。
N-linked glycans isolated from human plasma proteins have been profiled and sequenced by mass spectrometry using an ion trap instrument (ITMSn). The released glycans were prepared as reduced, methylated analogs and directly infused into a chip-based nanoelectrospray ionization system and analyzed by ITMSn. The resulting mass profiles (MS1) of IgG-depleted and non-depleted plasma samples were contrasted and these results were again compared with recent literature reports. Before depletion approximately 50 independent glycan ions were detected, this more than doubled to 106 after depletion. The mass range profiled was 1K-5KDa which included many doubly and triply charged ions that were resolved by higher MS resolution. Selected ions in the depleted sample were disassembled to define their detailed structure providing a high performance sequencing result. The simplicity of this non-chromatographic, direct infusion and gas-phase structural characterization compares most favorably with the latest reports using alternative instrumentation and adjunct techniques.
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影响因子：
2
作者：
Carre, Yoann;Klein, Andre;Morelle, Willy
通讯作者：
Morelle, Willy
影响因子：
4.4
作者：
Bereman MS;Young DD;Deiters A;Muddiman DC
通讯作者：
Muddiman DC
影响因子：
3.4
作者：
Omenn, GS;States, DJ;Hanash, SM
通讯作者：
Hanash, SM
在微流体芯片和飞行时间质谱上使用高性能液相色谱法的天然N连接聚糖结构的剖面。
影响因子：
3.4
作者：
Chu, Caroline S.;Ninonuevo, Milady R.;Clowers, Brian H.;Perkins, Patrick D.;An, Hyun Joo;Yin, Hongfeng;Killeen, Kevin;Miyamoto, Suzanne;Grimm, Rudolf;Lebrilla, Carlito B.
通讯作者：
Lebrilla, Carlito B.
影响因子：
--
作者：
Omtvedt, Lone A.;Royle, Louise;Rudd, Pauline A.
通讯作者：
Rudd, Pauline A.