Immunological Comparison of Shellfish Allergens by Competitive Enzyme-Linked Immunosorbent Assay
Immunological Comparison of Shellfish Allergens by Competitive Enzyme-Linked Immunosorbent Assay
复制标题
通过竞争性酶联免疫吸附测定法对贝类过敏原进行免疫学比较
DOI：
--
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Shiomi
中科院分区：
文献类型：
--
作者：
M. Ishikawa;Y. Nagashima;K. Shiomi
采用竞争性ELISA抑制分析法对牡蛎、虾夷扇贝、短颈蛤、角螺和海螺等5种贝类的过敏原进行了免疫学比较。贝类过敏原与牡蛎过敏原Crag 1之间存在交叉反应性，这表明，无论种类如何，贝类的主要过敏原是原肌球蛋白。当四个合成的重叠肽（P-1，LNRRIQLLEEDM; P-2，RIQLLEEDMERS; P-3，LLEEDMERSEER; P-4，EDMERSEERLQT），其中包括K21 d（为Crag 1提出的IgE结合表位，IQ LLEEDMERSEER）的一部分序列，进行竞争性ELISA抑制实验时，只有P-3抑制Crag I与致敏血清中特异性IgE抗体的结合。将K21 d的序列与四种合成肽的序列进行比较，发现K21 d的N端和C端区域都是与特异性IgE抗体结合所必需的。此外，P-3抑制扇贝和海螺过敏原以及牡蛎过敏原的特异性IgE抗体的结合，但基本上不从其他两个物种。因此得出结论，贝类过敏原并不总是在相同区域具有IgE结合表位，尽管它们都被认为是原肌球蛋白。
Allergens from five species of shellfishes (oyster Crassostrea gigas, scallop Patinopecten yessoensis, short-neck clam Tapes japonica, turban shell Turbo cornutus, and whelk Neptunea arthritica) were im munologically compared by competitive ELISA inhibition analyses. The cross-reactivity was recog nized between shellfish allergens and Crag 1, the oyster allergen, suggesting that, irrespective of spe cies, the major allergen in shellfishes is tropomyosin. When four synthesized overlapping peptides (P-1, LNRRIQLLEEDM; P-2, RIQLLEEDMERS; P-3, LLEEDMERSEER; P-4, EDMERSEERLQT), which comprehend a part of the sequence of K21d (IgE-binding epitope proposed for Crag 1, IQ LLEEDMERSEER), were subjected to competitive ELISA inhibition experiments, only P-3 inhibited the binding of Crag I to specific IgE antibodies in the sensitized serum. Comparison of the sequence of K21d with those of the four synthesized peptides revealed that both N and C-terminal regions of K21d are essential for the binding to specific IgE antibodies. In addition, P-3 inhibited the binding of specific IgE antibodies to scallop and whelk allergens as well as oyster allergens, but substantially not to those from the other two species. It was thus concluded that shellfish allergens do not always have IgE-bind ing epitopes in the same regions, although they are all thought to be tropomyosins.
影响因子：
2.8
作者：
DAUL, CB;SLATTERY, M;LEHRER, SB
通讯作者：
LEHRER, SB