In utero exposures to mint-flavored JUUL aerosol impair lung development and aggravate house dust mite-induced asthma in adult offspring mice.
In utero exposures to mint-flavored JUUL aerosol impair lung development and aggravate house dust mite-induced asthma in adult offspring mice.
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子宫内暴露于薄荷味的JUUL气雾剂损害肺发育，并加重成年后代小鼠的屋尘螨诱导的哮喘。
DOI：
10.1016/j.tox.2022.153272
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发表时间：
2022-07
期刊：
影响因子：
4.5
通讯作者：
Noel, Alexandra
中科院分区：
文献类型：
--
作者：
Cahill, Kerin M.;Johnson, Trenton K.;Perveen, Zakia;Schexnayder, Matthew;Xiao, Rui;Heffernan, Linda M.;Langohr, Ingeborg M.;Paulsen, Daniel B.;Penn, Arthur L.;Noel, Alexandra
关键词：
关于怀孕期间使用电子尼古丁递送系统(END)的报道很少，也没有关于产前暴露于JUUL的后代中哮喘的研究。在这里，我们测试了这样一种假设，即子宫内JUUL暴露会导致不良的分娩结局，并对成年后代产生持久的肺部健康影响。BALB/c母鼠在妊娠期间连续20天暴露于空气或薄荷味Juul气雾剂中，每天1小时。分别于出生后第0天(PND)或11周龄处死仔鼠，进行屋尘螨(HDM)攻击。评估了母鼠子宫/胎盘组织中的基因表达，并在PND0和11周龄时评估了后代的肺反应。暴露于JUUL的后代在PND0表现出体重和身长的减少。这些出生结局伴随着JUUL暴露的母鼠子宫/胎盘组织中与缺氧和氧化应激相关的54个基因的异常，以及子代肺中与Wnt信号相关的24个基因，加上9个与表观遗传学相关的基因，以及7个与炎症相关的基因。在11周龄时，与各自的空气+HDM对照组相比，JUUL+HDM暴露的小鼠表现出肺部炎症。此外，JUUL+HDM暴露导致与过敏和哮喘相关的几个基因调节失调。此外，JUUL+HDM雌性鼠的IL10ra基因启动子区域甲基化程度降低。综上所述，我们的小鼠模型显示，怀孕期间吸入Juul气雾剂会影响宫内环境，损害肺部发育，并增强以后生活中过敏性呼吸道反应的影响。
There are few reports concerning electronic nicotine delivery system (ENDS) use during pregnancy and no studies on asthma in prenatally JUUL-exposed offspring. Here, we tested the hypothesis that in utero JUUL exposure causes unfavorable birth outcomes and lasting pulmonary health effects in adult offspring. BALB/c dams were exposed to either air or mint-flavored JUUL aerosol, 1-hr/d, 20 consecutive days during gestation. Offspring were sacrificed on post-natal day (PND) 0 or at 11-week of age, following house dust mite (HDM) challenge. Gene expression was assessed in the uterine/placental tissue of the dams and lung responses were assessed in offspring at PND0 and at 11 weeks of age. JUUL-exposed offspring exhibited decreased body weights and lengths at PND0. These birth outcomes were accompanied by dysregulation of 54 genes associated with hypoxia and oxidative stress in the uterine/placental tissues of JUUL-exposed dams, as well as 24 genes in the lungs of the offspring related to Wnt signaling, plus 9 genes related to epigenetics, and 7 genes related to inflammation. At 11 weeks of age, JUUL + HDM exposed mice exhibited pulmonary inflammation when compared to their respective air + HDM controls. Additionally, the JUUL + HDM exposure dysregulated several genes associated with allergies and asthma. Further, the JUUL + HDM females showed decreased methylation of the promoter region of the Il10ra gene. Taken together, our mouse model shows that inhalation of JUUL aerosols during pregnancy affects the intrauterine environment, impairs lung development, and heightens the effects of allergic airway responses later in life.
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DOI：
10.1186/1477-7827-10-49
发表时间：
2012-06-29
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Agarwal A;Aponte-Mellado A;Premkumar BJ;Shaman A;Gupta S
通讯作者：
Gupta S
影响因子：
2.7
作者：
Branchfield K;Li R;Lungova V;Verheyden JM;McCulley D;Sun X
通讯作者：
Sun X
DOI：
10.1165/rcmb.2006-0116oc
发表时间：
2007-01-01
影响因子：
6.4
作者：
Bry, Kristina;Whitsett, Jeffrey A.;Lappalainen, Urpo
通讯作者：
Lappalainen, Urpo
影响因子：
2.4
作者：
Burton GJ
通讯作者：
Burton GJ
影响因子：
5.8
作者：
Conroy DM;Williams TJ
通讯作者：
Williams TJ