NanoSIMS imaging of extracellular electron transport processes during microbial iron(III) reduction.
NanoSIMS imaging of extracellular electron transport processes during microbial iron(III) reduction.
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DOI：
10.1093/femsec/fiy104
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发表时间：
2018-08-01
影响因子：
4.2
通讯作者：
Lloyd JR
中科院分区：
文献类型：
--
作者：
Newsome L;Lopez Adams R;Downie HF;Moore KL;Lloyd JR
微生物铁（III）还原可以对自然和工程环境中污染物的命运产生深远的影响。吉伯菌和希瓦氏菌利用不同的细胞外电子传递机制。还原不溶性Fe（III）矿物质。在这里，我们准备了一个薄膜的铁（III）-（oxyhydr）氧化物掺杂砷，并允许矿物涂层被定殖的Geophylla sulfreducens或希瓦氏ANA 3标记的13 C从有机电子供体。这保留了代谢活性Fe（III）还原细菌和它们呼吸的氧化铁（III）-（oxyhydr）氧化物之间的空间关系。NanoSIMS成像显示G. sulfurreducens与铁（III）-（oxyhydr）氧化物表面共定位，并显着更多的13 C-富集相比，远离矿物的细胞，与Geophysical物种需要直接接触细胞外电子受体，以支持生长一致。13 C富集的S. ANA 3和铁（III）-（oxyhydr）氧化物表面，与希瓦氏菌属物种能够使用分泌的内源性介质间接还原Fe（III）。结果表明，在G. sulfriducens相比，散装矿物，突出了这种类型的分析探测微生物细胞和铁微量金属分布之间的相互作用在地球化学实验的有用性。NanoSIMS成像显示，Geobactersulfurreducens的代谢活性细胞与铁（III）-（oxyhydr）氧化物表面共同定位，与这种需要与细胞外电子受体直接接触的细菌一致。
Microbial iron(III) reduction can have a profound effect on the fate of contaminants in natural and engineered environments. Different mechanisms of extracellular electron transport are used by Geobacter and Shewanella spp. to reduce insoluble Fe(III) minerals. Here we prepared a thin film of iron(III)-(oxyhydr)oxide doped with arsenic, and allowed the mineral coating to be colonised by Geobacter sulfurreducens or Shewanella ANA3 labelled with 13C from organic electron donors. This preserved the spatial relationship between metabolically active Fe(III)-reducing bacteria and the iron(III)-(oxyhydr)oxide that they were respiring. NanoSIMS imaging showed cells of G. sulfurreducens were co-located with the iron(III)-(oxyhydr)oxide surface and were significantly more 13C-enriched compared to cells located away from the mineral, consistent with Geobacter species requiring direct contact with an extracellular electron acceptor to support growth. There was no such intimate relationship between 13C-enriched S. ANA3 and the iron(III)-(oxyhydr)oxide surface, consistent with Shewanella species being able to reduce Fe(III) indirectly using a secreted endogenous mediator. Some differences were observed in the amount of As relative to Fe in the local environment of G. sulfurreducens compared to the bulk mineral, highlighting the usefulness of this type of analysis for probing interactions between microbial cells and Fe-trace metal distributions in biogeochemical experiments. NanoSIMS imaging shows that metabolically-active cells of Geobactersulfurreducens were co-located with an iron(III)-(oxyhydr)oxide surface, consistent with this bacterium requiring direct contact with an extracellular electron acceptor.
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影响因子：
5
作者：
Cutting, R. S.;Coker, V. S.;Vaughan, D. J.
通讯作者：
Vaughan, D. J.
DOI：
10.1073/pnas.0907468107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Harris, H. W.;El-Naggar, M. Y.;Nealson, K. H.
通讯作者：
Nealson, K. H.
影响因子：
64.8
作者：
Islam, FS;Gault, AG;Lloyd, JR
通讯作者：
Lloyd, JR
影响因子：
4.6
作者：
Edwards MJ;White GF;Norman M;Tome-Fernandez A;Ainsworth E;Shi L;Fredrickson JK;Zachara JM;Butt JN;Richardson DJ;Clarke TA
通讯作者：
Clarke TA
影响因子：
6.4
作者：
Gnanaprakasam ET;Lloyd JR;Boothman C;Ahmed KM;Choudhury I;Bostick BC;van Geen A;Mailloux BJ
通讯作者：
Mailloux BJ