Genomic and metabolomic insights into the natural product biosynthetic diversity of a feral-hog-associated Brevibacillus laterosporus strain.
Genomic and metabolomic insights into the natural product biosynthetic diversity of a feral-hog-associated Brevibacillus laterosporus strain.
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DOI：
10.1371/journal.pone.0090124
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cichewicz RH
中科院分区：
文献类型：
--
作者：
Theodore CM;Stamps BW;King JB;Price LS;Powell DR;Stevenson BS;Cichewicz RH
Bacteria associated with mammals are a rich source of microbial biodiversity; however, little is known concerning the abilities of these microbes to generate secondary metabolites. This report focuses on a bacterium isolated from the ear of a feral hog from southwestern Oklahoma, USA. The bacterium was identified as a new strain (PE36) of Brevibacillus latersporus, which was shown via genomic analysis to contain a large number of gene clusters presumably involved in secondary metabolite biosynthesis. A scale-up culture of B. latersporus PE36 yielded three bioactive compounds that inhibited the growth of methicillin-resistant Staphylococcus aureus (basiliskamides A and B and 12-methyltetradecanoic acid). Further studies of the isolate's secondary metabolome provided both new (auripyrazine) and previously-described pyrazine-containing compounds. In addition, a new peptidic natural product (auriporcine) was purified that was determined to be composed of a polyketide unit, two L-proline residues, two D-leucine residues, one L-leucine residue, and a reduced L-phenylalanine (L-phenylalanol). An examination of the genome revealed two gene clusters that are likely responsible for generating the basiliskamides and auriporcine. These combined genomic and chemical studies confirm that new and unusual secondary metabolites can be obtained from the bacterial associates of wild mammals.
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影响因子：
14.9
作者：
Blin K;Medema MH;Kazempour D;Fischbach MA;Breitling R;Takano E;Weber T
通讯作者：
Weber T
影响因子：
--
作者：
Jensen K;Niederkrüger H;Zimmermann K;Vagstad AL;Moldenhauer J;Brendel N;Frank S;Pöplau P;Kohlhaas C;Townsend CA;Oldiges M;Hertweck C;Piel J
通讯作者：
Piel J
影响因子：
3.7
作者：
Li K;Bihan M;Yooseph S;Methé BA
通讯作者：
Methé BA
影响因子：
5.1
作者：
Barsby, T;Kelly, MT;Andersen, RJ
通讯作者：
Andersen, RJ
影响因子：
2.5
作者：
Jessup, DA
通讯作者：
Jessup, DA