antiSMASH 2.0--a versatile platform for genome mining of secondary metabolite producers.
antiSMASH 2.0--a versatile platform for genome mining of secondary metabolite producers.
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DOI：
10.1093/nar/gkt449
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发表时间：
2013-07
影响因子：
14.9
通讯作者：
Weber T
中科院分区：
文献类型：
--
作者：
Blin K;Medema MH;Kazempour D;Fischbach MA;Breitling R;Takano E;Weber T
微生物次级代谢产物是抗生素和其他药物的有效来源。基因组挖掘其生物合成基因簇已成为加速其鉴定和表征的关键方法。2011年，我们开发了antiSMASH，这是一个基于Web的分析平台，可以自动化这一过程。在这里，我们介绍了高度改进的antiSMASH 2.0版本，可在http://antismash.secondarymetabolites.org/上获得。在新版本中，antiSMASH采用即插即用概念进行了完全重新设计，可以轻松集成新的预测器或输出模块。antiSMASH 2.0现在支持同时输入多个相关序列（multi-FASTA/GenBank/EMBL），这允许分析包含多个重叠群的基因组草案。此外，现在可以直接分析蛋白质序列。antiSMASH 2.0还具有检测其他次级代谢物类别的能力，包括寡糖抗生素、吩嗪、硫肽、高丝氨酸内酯、膦酸盐和呋喃。用于预测簇最终产物的核心结构的算法现在还包括lantipeptides，除了聚酮和非核糖体肽。antiSMASH AntiterBlast功能已扩展到识别参与特定化学结构单元生物合成的亚簇。这些新功能使antiSMASH 2.0成为鉴定和分析微生物中新型次级代谢物生物合成途径的最全面的资源。
Microbial secondary metabolites are a potent source of antibiotics and other pharmaceuticals. Genome mining of their biosynthetic gene clusters has become a key method to accelerate their identification and characterization. In 2011, we developed antiSMASH, a web-based analysis platform that automates this process. Here, we present the highly improved antiSMASH 2.0 release, available at http://antismash.secondarymetabolites.org/. For the new version, antiSMASH was entirely re-designed using a plug-and-play concept that allows easy integration of novel predictor or output modules. antiSMASH 2.0 now supports input of multiple related sequences simultaneously (multi-FASTA/GenBank/EMBL), which allows the analysis of draft genomes comprising multiple contigs. Moreover, direct analysis of protein sequences is now possible. antiSMASH 2.0 has also been equipped with the capacity to detect additional classes of secondary metabolites, including oligosaccharide antibiotics, phenazines, thiopeptides, homo-serine lactones, phosphonates and furans. The algorithm for predicting the core structure of the cluster end product is now also covering lantipeptides, in addition to polyketides and non-ribosomal peptides. The antiSMASH ClusterBlast functionality has been extended to identify sub-clusters involved in the biosynthesis of specific chemical building blocks. The new features currently make antiSMASH 2.0 the most comprehensive resource for identifying and analyzing novel secondary metabolite biosynthetic pathways in microorganisms.
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影响因子：
10.7
作者：
Medema MH;Takano E;Breitling R
通讯作者：
Breitling R
影响因子：
14.9
作者：
de Jong A;van Heel AJ;Kok J;Kuipers OP
通讯作者：
Kuipers OP
DOI：
10.1073/pnas.0700962104
发表时间：
2007-06-19
影响因子：
11.1
作者：
Udwary, Daniel W.;Zeigler, Lisa;Moore, Bradley S.
通讯作者：
Moore, Bradley S.
影响因子：
4.1
作者：
Weber, T.;Rausch, C.;Wohlleben, W.
通讯作者：
Wohlleben, W.
影响因子：
3.2
作者：
Ohnishi, Yasuo;Ishikawa, Jun;Horinouchi, Sueharu
通讯作者：
Horinouchi, Sueharu