Anthrax toxin receptor 2 promotes human uterine smooth muscle cell viability, migration and contractility.
Anthrax toxin receptor 2 promotes human uterine smooth muscle cell viability, migration and contractility.
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DOI：
10.1016/j.ajog.2013.09.030
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发表时间：
2014-02
影响因子：
9.8
通讯作者：
Reeves, Claire Vech
中科院分区：
文献类型：
--
作者：
Vink, Joy Yumiko;Charles-Horvath, Pelisa Cheryll;Kitajewski, Jan Krzysztof;Reeves, Claire Vech
关键词：
之前，我们证明了炭疽毒素受体2敲除（Antxr 2 −/−）小鼠具有生育能力，但无法在足月时分娩幼崽。这种分娩缺陷与子宫中细胞外基质（ECM）蛋白的过度积累和子宫肌层细胞含量减少有关。Antxr 2 −/−子宫组织中的子宫肌细胞丢失促使我们评估ANTXR 2是否对人类子宫平滑肌细胞（HUSMC）的活力和功能至关重要。我们对HUSMC进行慢病毒介导的敲低（R2 KD）或逆转录病毒介导的ANTXR 2过表达（R2 OE）。流式细胞术证实细胞系中的R2 KD或R2 OE与对照（CTL）相比。通过TUNEL法、Boyden小室法和催产素介导的胶原收缩法评价CTL、R2 KD和R2 OE细胞的细胞行为和功能。明胶酶谱法检测基质金属蛋白酶（MMP）活性。细胞系和样品一式两份运行。使用Student t检验进行统计分析。ANTXR 2由HUSMC表达。与CTL相比，HUMSC-R2 KD细胞表现出凋亡增加（p<0.05）和迁移减少（p<0.05），而HUSMC-R2 OE细胞表现出凋亡无变化（p=0.91）和迁移增加（p=0.05）。HUMSC-R2 KD细胞的收缩显著小于CTL，而HUSMC-R2 OE细胞的收缩性与CTL相比没有差异。与CTL相比，HUMSC-R2 KD细胞中MMP 2活性略微降低，而HUSMC-R2 OE细胞中MMP 2活性升高。ANTXR 2由HUSMC表达，并且似乎对正常HUSMC的活力、迁移和收缩性很重要。需要进一步的研究来阐明ANTXR 2是否对正常和异常分娩模式很重要。
Previously we demonstrated anthrax toxin receptor 2 knockout (Antxr2−/−) mice are fertile but fail to deliver their pups at term. This parturition defect is associated with over-accumulation of extracellular matrix (ECM) proteins and decreased myometrial cell content in the uterus. Myometrial cell loss in Antxr2−/− uterine tissue prompted us to evaluate if ANTXR2 is essential for human uterine smooth muscle cell (HUSMC) viability and function. We subjected HUSMC to lentiviral-mediated knock down (R2KD) or retroviral-mediated over-expression (R2OE) of ANTXR2. Flow cytometry confirmed R2KD or R2OE in cell lines vs control (CTL). Cell behavior and function in CTL, R2KD and R2OE cells were evaluated for apoptosis via TUNEL assay, migration via Boyden chamber assay and with oxytocin-mediated collagen contraction assays. Matrix metalloproteinase (MMP) activity was evaluated using gelatin zymography. Cell lines and samples were run in duplicate. Student t-test was used for statistical analysis. ANTXR2 is expressed by HUSMC. HUMSC-R2KD cells exhibited increased apoptosis (p<0.05) and decreased migration (p<0.05) while HUSMC-R2OE cells exhibited no change in apoptosis (p=0.91) and increased migration (p=0.05) vs CTL. HUMSC-R2KD cells contracted significantly less than CTL while HUSMC-R2OE cells showed no difference in contractility vs CTL. MMP2 activity appeared slightly decreased in HUMSC-R2KD cells and increased in HUSMC-R2OE cells vs CTL. ANTXR2 is expressed by HUSMC and appears important for normal HUSMC viability, migration and contractility. Further studies are needed to delineate if ANTXR2 is important for normal and abnormal labor patterns.
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DOI：
10.1016/j.bbamcr.2010.11.013
发表时间：
2011-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Yang MY;Chaudhary A;Seaman S;Dunty J;Stevens J;Elzarrad MK;Frankel AE;St Croix B
通讯作者：
St Croix B
DOI：
10.1073/pnas.0431098100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Scobie, HM;Rainey, GJA;Young, JAT
通讯作者：
Young, JAT
影响因子：
4.8
作者：
ROSSI, MJ;CHEGINI, N;MASTERSON, BJ
通讯作者：
MASTERSON, BJ
DOI：
10.1165/ajrcmb.25.5.4605
发表时间：
2001-11-01
影响因子：
6.4
作者：
Freyer, AM;Johnson, SR;Hall, IP
通讯作者：
Hall, IP
影响因子：
9.8
作者：
Dowling, O;Difeo, A;Martignetti, JA
通讯作者：
Martignetti, JA