FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2.
FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2.
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FIF [成纤维细胞生长因子-2 (FGF-2) 相互作用因子] 是一种核推定抗凋亡因子，与 FGF-2 特异性相互作用。
DOI：
10.1210/mend.14.11.0556
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发表时间：
2000
影响因子：
--
通讯作者：
Béatrix Bugler
中科院分区：
文献类型：
--
作者：
Loïc Van Den Berghe;Henrik Laurell;I. Huez;C. Zanibellato;Hervé Prats;Béatrix Bugler
大量证据表明，成纤维细胞生长因子2 （FGF-2）的一些活性依赖于细胞内的作用模式。最近，我们发现FGF-2的三种高分子质量（HMM）核形式是320 kda蛋白复合物的一部分，而细胞质aug启动形式包含在130 kda复合物中。因此，FGF内源性靶点的表征对于阐明其内源性活性变得至关重要。通过筛选基于gal4的酵母双杂交表达文库，我们分离出一个编码核蛋白55 kDa的基因，FIF （FGF-2相互作用因子），它特异性地与FGF-2相互作用，但不与FGF-1、FGF-3或FGF-6相互作用。在该系统中，FIF与nh2扩展的24 kda FGF形式的相互作用同样良好，这表明FIF结合基序位于FGF-2的最后155个氨基酸中。然而，共免疫沉淀实验显示与HMM FGF-2独家相关。预测的蛋白质包含一个典型的亮氨酸拉链结构域和三个重叠的疏水七重复。跨越这些重复序列的区域，以及位于FIF蛋白n端部分的区域，与FGF-2的结合有关。与全长FIF蛋白相比，几种缺失结构能够反激活lac-Z报告基因。此外，cooh末端部分，而不是全长FIF蛋白，先前已被证明具有抗凋亡特性。因此，我们讨论了这些活性可能通过FGF-2相互作用反映FIF的生理功能的可能性。
Numerous evidence indicates that some of the activities of fibroblast growth factor 2 (FGF-2) depend on an intracrine mode of action. Recently, we showed that three high molecular mass (HMM) nuclear forms of FGF-2 are part of a 320-kDa protein complex while the cytoplasmic AUG-initiated form is included in a 130-kDa complex. Consequently, the characterization of FGF endogenous targets has become crucial to allow the elucidation of their endogenous activities. Through the screening of GAL4-based yeast two-hybrid expression libraries, we have isolated a gene encoding a nuclear protein of 55 kDa, FIF (FGF-2-interacting-factor), which interacts specifically with FGF-2 but not with FGF-1, FGF-3, or FGF-6. In this system, FIF interacts equally well with the NH2-extended 24-kDa FGF form as with the 18-kDa form, indicating that the FIF-binding motif is located in the last 155 amino acids of FGF-2. Nevertheless, coimmunoprecipitation experiments showed an exclusive association with HMM FGF-2. The predicted protein contains a canonical leucine zipper domain and three overlapping hydrophobic heptad repeats. The region spanning these repeats is, together with a region located in the N-terminal part of the FIF protein, implicated in the binding to FGF-2. In contrast to the full-length FIF protein, several deletion constructs were able to transactivate a lac-Z reporter gene. Furthermore, the COOH-terminal part, but not the full-length FIF protein, has previously been shown to exhibit antiapoptotic properties. Thus we discuss the possibility that these activities could reflect a physiological function of FIF through its interaction with FGF-2.
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影响因子：
4
作者：
Jane Reiland;A. Rapraeger
通讯作者：
Jane Reiland;A. Rapraeger
胰岛素样生长因子 1 或成纤维细胞生长因子 2 抑制骨骼肌分化需要丝裂原激活蛋白激酶激酶 (MEK) 活性。
DOI：
10.1210/endo.139.4.5950
发表时间：
1998
期刊：
Endocrinology.
影响因子：
--
作者：
Weyman,CM;Wolfman,A
通讯作者：
Wolfman,A
影响因子：
56.9
作者：
IMAMURA, T;ENGLEKA, K;MACIAG, T
通讯作者：
MACIAG, T
影响因子：
--
作者：
Daniel E. Johnson;L. Williams
通讯作者：
Daniel E. Johnson;L. Williams
影响因子：
10.5
作者：
DURFEE, T;BECHERER, K;ELLEDGE, SJ
通讯作者：
ELLEDGE, SJ