LabEmbryoCam: An opensource phenotyping system for developing aquatic animals
LabEmbryoCam: An opensource phenotyping system for developing aquatic animals
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LabEmbryoCam：用于开发水生动物的开源表型系统
DOI：
10.1101/2023.04.11.536373
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Ibbini Z
中科院分区：
文献类型：
--
作者：
Ibbini Z
表型组学是在个人范围内获取高维数据，在与人类健康相关的生物研究领域，包括医学和作物科学，正在被证明是变革性的。然而，更广泛地说，与分子组学形成鲜明对比的是，缺乏可转让的可转让技术和研究方法大大阻碍了对表型组学的吸收，可转让技术一直是分子组学的重要推动因素。水生胚胎因其体积小、分类多样性、生态相关性以及高度的时间、空间和功能变化而成为表型组学的天然模型。在这里，我们介绍了LabEmbryoCam，这是一个自主的表型平台，用于对以多孔平板格式培养的发育中的水生胚胎进行时间流逝成像，虽然该仪器针对胚胎进行了优化，但它的通用性非常强。LabEmbryoCam利用了3D打印、单板电脑、消费电子产品和步进电机驱动的运动功能。我们将这些整合到一个紧凑而模块化的实验室设备中，以提供相机和镜头的X、Y和Z运动，简化的Web应用程序，用于快速设置实验，用户电子邮件通知和加湿室，以减少长时间采集过程中的蒸发。提供下游分析，支持自动胚胎分割、心率测量、运动跟踪和能量替代特性(EPT)测量。LabEmbryoCam是一种可扩展、灵活的实验室仪器，它利用胚胎和生命早期生物来应对关键的全球挑战，包括生物敏感性评估、毒理学筛查，而且还支持更广泛地参与生命早期阶段的工作。
Phenomics is the acquisition of high-dimensional data on an individual-wide scale and is proving transformational in areas of biological research related to human health including medicine and the crop sciences. However, more broadly, a lack of accessible transferrable technologies and research approaches is significantly hindering the uptake of phenomics, in contrast to molecular-omics for which transferrable technologies have been a significant enabler. Aquatic embryos are natural models for phenomics, due to their small size, taxonomic diversity, ecological relevance, and high levels of temporal, spatial and functional change. Here, we present LabEmbryoCam, an autonomous phenotyping platform for timelapse imaging of developing aquatic embryos cultured in a multiwell plate format, and while optimised for embryos, the instrument is extremely versatile. The LabEmbryoCam capitalises on 3D printing, single board computers, consumer electronics and stepper motor enabled motion. We combine these into a compact and modular laboratory insturment to provide X, Y and Z motion of a camera and lens, a web application streamlined for rapid setup of experiments, user email notifications and a humidification chamber to reduce evaporation over prolonged acquisitions. Downstream analyses are provided, enabling automated embryo segmentation, heartrate measurement, motion tracking, and energy proxy trait (EPT) measurement. The LabEmbryoCam is a scalable, and flexible laboratory instrument, that leverages embryonic and early life stage organisms to tackle key global challenges including biological sensitivity assessment, toxicological screening, but also to support broader engagement with the earliest stages of life.
亲子发育事件时间的相似性：异时性的起源？
DOI：
--
发表时间：
2013
期刊：
Proceedings of the Royal Society B: Biological Sciences
影响因子：
--
作者：
O. Tills;S. Rundle;J. Spicer
通讯作者：
J. Spicer
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert
热生理学的差异可能有助于潮间带生态型的垂直分离
影响因子：
1.6
作者：
Dwane C
通讯作者：
Dwane C