Deinococcus petrolearius sp. nov. isolated from crude oil recovery water in China
Deinococcus petrolearius sp. nov. isolated from crude oil recovery water in China
复制标题
石油奇球菌 sp.
DOI：
10.1007/s10482-017-0957-9
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发表时间：
2017-10
影响因子：
2.6
通讯作者：
Liu Jianguo
中科院分区：
文献类型：
--
作者：
Xi Lijun;Qiao Nenghu;Zhang Jingjing;Li Jing;Liu Dejian;You Jing;Liu Jianguo
从华北油田采出水中分离到一株革兰氏染色阳性、非运动、球形、红色、兼性厌氧细菌，命名为6.1T。新菌株表现出耐紫外线照射（> 1000 J m-2）。16 SrRNA基因序列分析表明，菌株6.1T与Deinococcus citri DSM 24791 T和Deinococcus gobiensis I-0 T的相似性分别为98.1%和97.8%，与其它近缘类群的相似性均低于93.5%。主要的细胞脂肪酸被鉴定为总特征3（C16：1ω 7 c和/或iso-C15：02-OH），其次是iso-C17：1ω 9 c和C16：0。发现极性脂质谱含有磷脂、糖脂、磷酸糖脂和氨基磷脂。主要的呼吸醌被鉴定为MK-8。DNA G + C含量测定为68.3mol%。菌株6.1T与D. Citri DSM 24791 T的抗性为45.6 ± 7.1%;戈壁虎I-OT为36.6 ± 4.7%。根据系统发育、化学分类和表型分析，我们认为菌株6.1T代表异常球菌属的一个新种，并将其命名为Deinococcus petrolearius sp. nov.
A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1T, was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m-2). Based on 16S rRNA gene sequence comparisons, strain 6.1Tshows high similarity to Deinococcus citri DSM 24791T(98.1%) and Deinococcus gobiensis I-0T(97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C16:1ω7c and/or iso-C15:02-OH), followed by iso-C17:1ω9c and C16:0. The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA-DNA hybridization between strain 6.1Tand D. citri DSM 24791Twas 45.6 ± 7.1% and with D. gobiensis I-OTwas 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1Trepresents a novel species of the genus Deinococcus, for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1T(= CGMCC 1.15053T= KCTC 33744T).
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影响因子：
2.1
作者：
B. Tindall
通讯作者：
B. Tindall
DOI：
10.1007/s10482-017-0841-7
发表时间：
2017-02
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
R. Rosselló-Móra;M. Trujillo;I. Sutcliffe
通讯作者：
R. Rosselló-Móra;M. Trujillo;I. Sutcliffe
DOI：
10.1099/00221287-100-2-221
发表时间：
1977-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
COLLINS, MD;PIROUZ, T;MINNIKIN, DE
通讯作者：
MINNIKIN, DE
DOI：
--
发表时间：
2008
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro