Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation.
Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation.
复制标题
蓝藻类囊体膜复合物超分子结构在远红光光适应过程中的变化
DOI：
10.1126/sciadv.abj4437
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发表时间：
2022-02-11
期刊：
影响因子：
13.6
通讯作者：
Hunter CN
中科院分区：
文献类型：
--
作者：
MacGregor-Chatwin C;Nürnberg DJ;Jackson PJ;Vasilev C;Hitchcock A;Ho MY;Shen G;Gisriel CJ;Wood WHJ;Mahbub M;Selinger VM;Johnson MP;Dickman MJ;Rutherford AW;Bryant DA;Hunter CN
蓝细菌在自然界中无处不在，并且已经开发了许多策略，使它们能够生活在各种环境中。某些蓝藻合成叶绿素d和f，以适应富含远红光（FRL）的生态位，并在一个称为FRL诱导的光适应（FaRLiP）的过程中将旁系同源光合蛋白纳入其光合器官。我们的特点是参与FRL驱动的光合作用的大分子变化，并使用原子力显微镜检查超分子组织的光系统I与FaRLiP在三个蓝藻物种。质谱分析显示了伴随FaRLiP的热色球藻PCC 7203蛋白质组的变化。荧光寿命成像显微镜和电子显微镜揭示了光系统复合物的细胞分布的改变，并说明了FaRLiP响应的细胞间变异性。远红光光适应改变了蓝藻类囊体膜中光系统I的寡聚状态。
Cyanobacteria are ubiquitous in nature and have developed numerous strategies that allow them to live in a diverse range of environments. Certain cyanobacteria synthesize chlorophylls d and f to acclimate to niches enriched in far-red light (FRL) and incorporate paralogous photosynthetic proteins into their photosynthetic apparatus in a process called FRL-induced photoacclimation (FaRLiP). We characterized the macromolecular changes involved in FRL-driven photosynthesis and used atomic force microscopy to examine the supramolecular organization of photosystem I associated with FaRLiP in three cyanobacterial species. Mass spectrometry showed the changes in the proteome of Chroococcidiopsis thermalis PCC 7203 that accompany FaRLiP. Fluorescence lifetime imaging microscopy and electron microscopy reveal an altered cellular distribution of photosystem complexes and illustrate the cell-to-cell variability of the FaRLiP response. Photoacclimation to far-red light changes the oligomeric state of photosystem I in cyanobacterial thylakoid membranes.
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