Insights on the aerobic biodegradation of agricultural wastes under simulated rapid composting conditions
Insights on the aerobic biodegradation of agricultural wastes under simulated rapid composting conditions
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模拟快速堆肥条件下农业废弃物好氧生物降解的见解
DOI：
10.1016/j.jclepro.2019.02.163
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发表时间：
2019-05
影响因子：
11.1
通讯作者：
Shen Qirong
中科院分区：
文献类型：
--
作者：
Wang Mengmeng;Ma Lei;Kong Zhijiang;Wang Quan;Fang Lin;Liu Dongyang;Shen Qirong
了解快速堆肥过程中有机物的转化规律是理解生物降解过程的基础。本文采用原子力显微镜（AFM）、X射线光电子能谱（XPS）和13 C核磁共振谱（13 C NMR）等技术对生物降解过程中基质的组成和结构进行了研究。基质的总碳含量比初始含量降低了27.7%，而总氮含量从1.1%增加到1.8%。3 ~ 14 d是堆肥过程的活跃期，水溶性碳、水溶性氮和水解酶活性波动较大。微纤维和深沟的出现表明，隧道效应可能是堆肥微生物降解木质纤维素的机制之一。X射线光电子能谱（XPS）分析结果表明，可降解的主要碳组分为C1（含C-H或C-C键），比初始底物减少了25.5%。~（13）C NMR分析表明，在所有处理条件下，O-alkyl-C基团占共振信号的76.6%~ 83.1%，alkyl-C在8.1%~ 10.2%之间波动，表明木质纤维素的有效降解。综上所述，结合原子力显微镜（AFM）的形态学观察和XPS、13 C NMR的化学分析，是表征农业废弃物生物降解过程的一种很有前途的方法。
Knowledge of organic matter transformation under simulated rapid composting is essential for the understanding of biodegradation processes. Herein, several technologies, including atomic force microscope (AFM), X-ray photoelectron spectroscopy (XPS) and13C Nuclear Magnetic Resonance Spectroscopy (13C NMR) were used to gain insight of the composition and architecture of the substrate during biodegradation. The total carbon of the substrate was decreased by 27.7% compared to the initial content, while the total nitrogen was increased from 1.1% to 1.8% at the end. The water soluble carbon, water soluble nitrogen and hydrolytic enzyme activities fluctuated strongly between 3 and 14 days, which was considered as the active stage of the composting process. The appearance of microfibers and deep ditch indicated that the tunneling might be one of the mechanisms of the composting microorganisms to degrade the lignocellulose. The X-ray photoelectron spectroscopy (XPS) analysis results showed that the main degradable carbon component was C1 (containing C-H or C-C bonds), which was decreased by 25.5% compared to the initial substrate. The13C NMR analysis showed that the O-alkyl-C group dominated the resonance signals in all treatments ranging from 76.6% to 83.1% and alkyl-C fluctuated from 8.1% to 10.2%, indicating the effective degradation of lignocellulose. In summary, the combination of morphological observations by atomic force microscope (AFM) and the chemical analysis by XPS and13C NMR is a promising approach for the characterization of agricultural wastes biodegradation process.
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影响因子：
11.4
作者：
Xueqin Wang;Hongyang Cui;Jianhong Shi;Xinyu Zhao;Yue Zhao;Zi-min Wei
通讯作者：
Xueqin Wang;Hongyang Cui;Jianhong Shi;Xinyu Zhao;Yue Zhao;Zi-min Wei
影响因子：
11.4
作者：
L. Zhang;Jiachao Zhang;G. Zeng;Haoran Dong;Yaoning Chen;Chao Huang;Yuan Zhu;Rui Xu;Yujun Cheng;Kunjie Hou;Weicheng Cao;Wei Fang
通讯作者：
L. Zhang;Jiachao Zhang;G. Zeng;Haoran Dong;Yaoning Chen;Chao Huang;Yuan Zhu;Rui Xu;Yujun Cheng;Kunjie Hou;Weicheng Cao;Wei Fang
影响因子：
5.8
作者：
Chen, Yaoning;Huang, Jingxia;Zhou, Wei
通讯作者：
Zhou, Wei
影响因子：
11.4
作者：
Ververis, C.;Georghiou, K.;Corleti, V.
通讯作者：
Corleti, V.
DOI：
10.1042/bj3530117
发表时间：
2001
期刊：
The Biochemical journal
影响因子：
--
作者：
Neil J. Parry;David E. Beever;Emyr Owen;Isabel Vandenberghe;Jozef Van Beeumen;M. K. Bhat
通讯作者：
Neil J. Parry;David E. Beever;Emyr Owen;Isabel Vandenberghe;Jozef Van Beeumen;M. K. Bhat