High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions.
High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions.
复制标题
DOI：
10.1093/nar/gky410
复制
发表时间：
2018-08-21
影响因子：
14.9
通讯作者：
Callaghan AJ
中科院分区：
文献类型：
--
作者：
Phillips JO;Butt LE;Henderson CA;Devonshire M;Healy J;Conway SJ;Locker N;Pickford AR;Vincent HA;Callaghan AJ
我们才刚刚开始解开非编码RNA参与的无数相互作用。复杂的RNA相互作用组是许多生物过程的基础，包括细菌毒力和人类疾病，并代表了开发潜在治疗干预措施的未开发资源。然而，从细胞内众多可能的结合伴侣中鉴定给定RNA的特异性缔合需要强大的高通量系统进行快速筛选。在这里，我们提出了第一次演示的功能RNA阵列作为一种新的平台技术，设计用于研究这种相互作用，使用固定的，活性的RNA。我们已经产生了高密度的RNA阵列的创新方法，涉及在体外转录的RNA的表面捕获。这种方法相对于现有技术具有显著的优势，特别是在结合伴侣特性方面的多功能性。事实上，证明了RNA阵列对RNA-小分子和RNA-RNA配对的原理应用，突出了它们作为基于RNA的网络映射和药物筛选的平台技术的潜力。此外，该方法的简单性支持比当前可用技术更大的用户可访问性。我们预计，功能性RNA阵列将在不断扩大的RNA表征领域中找到广泛的用途。
We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilized, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA–small molecule and RNA–RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greater user-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterization.
登录
查看更多内容
影响因子：
64.5
作者：
Pardee K;Green AA;Ferrante T;Cameron DE;DaleyKeyser A;Yin P;Collins JJ
通讯作者：
Collins JJ
影响因子：
3.7
作者：
Svensson, Sarah L.;Sharma, Cynthia M.
通讯作者：
Sharma, Cynthia M.
影响因子：
64.5
作者：
Pardee, Keith;Green, Alexander A.;Collins, James J.
通讯作者：
Collins, James J.
影响因子：
4.5
作者：
Srisawat, C;Engelke, DR
通讯作者：
Engelke, DR
DOI：
10.1073/pnas.1618370114
发表时间：
2017-04-04
影响因子：
11.1
作者：
She, Richard;Chakravarty, Anupam K.;Greenleaf, William J.
通讯作者：
Greenleaf, William J.