Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome.
Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome.
复制标题
DOI：
10.1093/nar/gki925
复制
发表时间：
2005
影响因子：
14.9
通讯作者：
Tsunewaki, K
中科院分区：
文献类型：
--
作者：
Ogihara, Y;Yamazaki, Y;Murai, K;Kanno, A;Terachi, T;Shiina, T;Miyashita, N;Nasuda, S;Nakamura, C;Mori, N;Takumi, S;Murata, M;Futo, S;Tsunewaki, K
应用一种新的基于基因的策略对小麦线粒体基因组进行测序，结果表明其结构为一个452 - 528 bp的环状分子，并提供了分子内重组的核苷酸水平证据。已经鉴定了单、相互和双重组产物，以及介导其形成的重复序列的核苷酸序列。基因组有55个基因，包括35个蛋白质编码基因、3个rRNA基因和17个tRNA基因。首次测定了7个小麦基因的核苷酸序列。9个基因具有外显子-内含子结构。负责生产多拷贝线粒体基因的基因扩增，一般来说，是物种特异性的，这表明这些基因的最近起源。基因（含内含子）、开放阅读框、重复序列、叶绿体和反转录元件来源的小麦线粒体DNA（mtDNA）分别占16%、17%、15%、3.0%和0.2%。小麦线粒体基因图谱的基因顺序与水稻和玉米图谱的基因顺序几乎没有同线性，表明在物种形成过程中发生了彻底的基因改组。与水稻和玉米相比，小麦中几乎所有独特的mtDNA序列都是冗余的。讨论了基于基因的策略的特点，并提出了线粒体基因扩增的机理模型。
The application of a new gene-based strategy for sequencing the wheat mitochondrial genome shows its structure to be a 452 528 bp circular molecule, and provides nucleotide-level evidence of intra-molecular recombination. Single, reciprocal and double recombinant products, and the nucleotide sequences of the repeats that mediate their formation have been identified. The genome has 55 genes with exons, including 35 protein-coding, 3 rRNA and 17 tRNA genes. Nucleotide sequences of seven wheat genes have been determined here for the first time. Nine genes have an exon–intron structure. Gene amplification responsible for the production of multicopy mitochondrial genes, in general, is species-specific, suggesting the recent origin of these genes. About 16, 17, 15, 3.0 and 0.2% of wheat mitochondrial DNA (mtDNA) may be of genic (including introns), open reading frame, repetitive sequence, chloroplast and retro-element origin, respectively. The gene order of the wheat mitochondrial gene map shows little synteny to the rice and maize maps, indicative that thorough gene shuffling occurred during speciation. Almost all unique mtDNA sequences of wheat, as compared with rice and maize mtDNAs, are redundant DNA. Features of the gene-based strategy are discussed, and a mechanistic model of mitochondrial gene amplification is proposed.
登录
查看更多内容
DOI：
10.1007/bf02464880
发表时间：
1989-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HIRATSUKA, J;SHIMADA, H;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
56.9
作者：
MILLER, RJ;KOEPPE, DE
通讯作者：
KOEPPE, DE
影响因子：
14.9
作者：
Handa, H
通讯作者：
Handa, H
影响因子：
3.5
作者：
BONEN, L;BIRD, S
通讯作者：
BIRD, S
影响因子：
11.4
作者：
FALCONET, D;LEJEUNE, B;GRAY, MW
通讯作者：
GRAY, MW