The relationship between odontoblasts and immunocompetent cells during dentinogenesis in rat incisors: an immunohistochemical study using OX6-monoclonal antibody.
The relationship between odontoblasts and immunocompetent cells during dentinogenesis in rat incisors: an immunohistochemical study using OX6-monoclonal antibody.
复制标题
大鼠门牙牙本质发生过程中成牙本质细胞和免疫活性细胞之间的关系：使用 OX6-单克隆抗体的免疫组织化学研究。
DOI：
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发表时间：
1994
影响因子：
--
通讯作者：
Yoshiro Takano
中科院分区：
文献类型：
--
作者：
Hayato Ohshima;Ichiro Kawahara;Takeyasu Maeda;Yoshiro Takano
采用OX6单克隆抗体进行免疫组织化学研究，研究了大鼠下切牙牙本质形成过程中成牙本质细胞与II类主要组织相容性复合物（MHC）抗原表达细胞之间的关系。牙髓含有大量 OX6 免疫阳性细胞，在生理条件下其形态从树突状到纺锤状各不相同。在电子显微镜下，这些免疫阳性细胞具有共同的细胞质特征，即细胞质中具有多泡体和特征性的细管泡结构。在牙本质发生的早期阶段，OX6 免疫阳性细胞（可能是未成熟类型）位于牙本质细胞下层。在活跃的牙本质形成过程中，OX6免疫阳性细胞数量增加并出现在成牙本质细胞层中，与靠近前牙本质的有孔毛细血管密切相关。这些细胞显示出树突状外观，并具有各种大小的多泡体和特征性的细管泡结构，但从未包含典型的吞噬体。另一方面，以典型吞噬体为特征的免疫阳性巨噬细胞倾向于占据牙髓的中心部分。结果表明，大多数（如果不是全部）位于成牙本质细胞层深处的 OX6 免疫阳性细胞是树突状细胞，在牙髓防御系统中发挥作用，抵抗通过有孔毛细血管从循环中到达的抗原分子。牙齿切端附近出现的 OX6 免疫阳性或免疫阴性巨噬细胞数量不断增加，被认为与退化成牙本质细胞的消除有关。
The relationship between odontoblasts and class II major histocompatibility complex (MHC) antigen-expressing cells in the process of dentinogenesis was studied in rat lower incisors, employing immunohistochemistry using OX6-monoclonal antibody. The dental pulp contained numerous OX6-immunopositive cells that varied in morphology from dendritic to spindle under physiological conditions. Under the electron microscope, these immunopositive cells shared common cytoplasmic features, i.e., multivesicular bodies and characteristic fine tubulovesicular structures in their cytoplasm. At the early stage of dentinogenesis, OX6-immunopositive cells, presumably of the immature type, were located in the subodontoblastic layer. During active dentin formation, the OX6-immunopositive cells increased in number and appeared in the odontoblast layer, associating intimately with fenestrated capillaries situated close to the predentin. These cells showed a dendritic appearance and possessed various sizes of multivesicular bodies and characteristic fine tubulovesicular structures, but never contained typical phagosomes. On the other hand, immunopositive macrophages characterized by typical phagosomes tended to occupy the central portion of the pulp. The results suggest that most, if not all, OX6-immunopositive cells situated deep in the odontoblast layer are dendritic cells playing a role in the defense system of the dental pulp against antigenic molecules arriving from the circulation via the fenestrated capillaries. The increasing number of OX6-immunopositive or immunonegative macrophages appearing near the incisal end of the tooth is thought to be involved in the elimination of degenerated odontoblasts.
血管周围树突状巨噬细胞作为人真皮微血管单元的免疫生物学成分。
DOI：
10.1111/1523-1747.ep12581078
发表时间：
1989
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Sontheimer,RD
通讯作者：
Sontheimer,RD