Detection of antibodies against human platelet antigens 15a and 15b by using a cell line panel
Detection of antibodies against human platelet antigens 15a and 15b by using a cell line panel
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使用细胞系组检测抗人血小板抗原 15a 和 15b 的抗体
DOI：
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发表时间：
2010
影响因子：
6.5
通讯作者：
F. Hirayama
中科院分区：
文献类型：
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作者：
Tomoya Hayashi;E. Amakishi;N. Matsuyama;K. Yasui;R. Furuta;Y. Hori;Y. Fukumori;Shigenori Tanaka;B. Curtis;M. Inoue;F. Hirayama
血小板表面膜含有多种分子，包括ABO血型抗原（Curtis et al，2000）、人白细胞抗原（HLA）、人血小板抗原（HPAs）和Nak抗原。这些分子的抗体被认为是血小板输注引起的各种反应的主要原因，如难治性和输血后紫癜。这些抗体也被认为可引起新生儿同种免疫性血小板减少症和特发性血小板减少性紫癜（Smith et al，1995; Bordin et al，1997）。HPA-15（Gov）同种异体抗原系统位于CD 109蛋白上，CD 109蛋白是一种175 kDa的糖基磷脂酰肌醇连接的糖蛋白，存在于几种组织上，也存在于造血干细胞、祖细胞和活化血小板和T细胞的亚群上。HPA-15等位基因的差异在于CD 109 cDNA编码区第2108位的A/C单核苷酸多态性，导致CD 109氨基酸682处的Tyr/Ser置换（Schuh et al，2002）。尽管人种之间存在差异，但HPA-15 a和HPA-15 b抗原的基因型频率大致相等（Cardone et al，2004）。
The platelet surface membrane contains a variety of molecules, including ABO blood type antigens (Curtis et al, 2000), human leucocyte antigens (HLAs), human platelet antigens (HPAs) and Nak antigen. Antibodies to these molecules have been regarded as the principal causes of various reactions elicited by platelet transfusion, such as refractoriness and post-transfusion purpura. These antibodies are also thought to cause neonatal alloimmune thrombocytopenia and idiopathic thrombocytopenic purpura (Smith et al, 1995; Bordin et al, 1997). The HPA-15 (Gov) alloantigen system is localized on the CD109 protein, a glycosylphosphatidylinositol-linked glycoprotein of 175 kDa that is found on several tissues and also on a subset of haematopoietic stem cells, progenitor cells and activated platelets and T cells. HPA-15 alleles differ by an A/C single nucleotide polymorphism at position 2108 of the coding region of CD109 cDNA, resulting in a Tyr/Ser substitution at CD109 amino acid 682 (Schuh et al, 2002). Despite differences between races, the genotypic frequencies of HPA-15a and HPA-15b antigens are roughly equal (Cardone et al, 2004).
影响因子：
20.3
作者：
Curtis, BR;Edwards, JT;Aster, RH
通讯作者：
Aster, RH