Behaviour of telomere and telomerase during aging and regeneration in zebrafish.
Behaviour of telomere and telomerase during aging and regeneration in zebrafish.
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DOI：
10.1371/journal.pone.0016955
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发表时间：
2011-02-09
期刊：
影响因子：
3.7
通讯作者：
Cayuela ML
中科院分区：
文献类型：
--
作者：
Anchelin M;Murcia L;Alcaraz-Pérez F;García-Navarro EM;Cayuela ML
端粒长度和端粒酶活性是人类疾病病理生物学中的重要因素。端粒相关疾病和早衰综合征的特征在于短端粒，这可能损害细胞活力，而肿瘤细胞可以通过异常上调端粒酶来防止端粒丢失。斑马鱼（Danio rerio）提供了多个实验操作的优势，比其他脊椎动物模型，因此，它最近被认为是一个潜在的模型衰老，癌症和再生研究。然而，它只被部分利用来阐明这些基本的生物过程。本研究的目的是，因此，调查端粒长度和端粒酶的表达和活性在不同品系的斑马鱼从不同的股票中心，以确定他们是否经历任何变化，在老化和再生。我们发现，虽然端粒酶的表达和端粒长度增加，从胚胎到成年阶段，他们急剧下降，尽管端粒酶活性检测在不同组织的老鱼。此外，我们观察到较弱的端粒酶表达上调再生鳍的老鱼，这与他们受损的再生能力。引人注目的是，端粒在所有年龄段的鳍再生过程中和反复截肢后被延长或维持，可能支持高细胞增殖率。我们的结论是，端粒酶的表达和端粒长度是密切相关的整个生命周期的鱼，这两个参数可以作为生物标志物的衰老在斑马鱼。我们的研究结果还揭示了端粒酶的表达，端粒长度和组织再生效率之间的直接关系。
Telomere length and telomerase activity are important factors in the pathobiology of human diseases. Age-related diseases and premature aging syndromes are characterized by short telomeres, which can compromise cell viability, whereas tumour cells can prevent telomere loss by aberrantly upregulating telomerase. The zebrafish (Danio rerio) offers multiple experimental manipulation advantages over other vertebrate models and, therefore, it has been recently considered as a potential model for aging, cancer, and regeneration studies. However, it has only partially been exploited to shed light on these fundamental biological processes. The aim of this study was, therefore, to investigate telomere length and telomerase expression and activity in different strains of zebrafish obtained from different stock centres to determine whether they undergo any changes during aging and regeneration. We found that although both telomerase expression and telomere length increased from embryo to adulthood stages, they drastically declined in aged fish despite telomerase activity was detected in different tissues of old fish. In addition, we observed a weaker upregulation of telomerase expression in regenerating fins of old fish, which well correlates with their impaired regeneration capacity. Strikingly, telomeres were elongated or maintained during the fin regeneration process at all ages and after repeated amputations, likely to support high cell proliferation rates. We conclude that the expression of telomerase and telomere length are closely related during the entire life cycle of the fish and that these two parameters can be used as biomarkers of aging in zebrafish. Our results also reveal a direct relationship between the expression of telomerase, telomere length and the efficiency of tissue regeneration.
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