Direct visualization of the interaction between pilin and exopolysaccharides of Myxococcus xanthus with eGFP-fused PilA protein.
Direct visualization of the interaction between pilin and exopolysaccharides of Myxococcus xanthus with eGFP-fused PilA protein.
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DOI：
10.1111/j.1574-6968.2011.02430.x
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发表时间：
2012-01
影响因子：
2.1
通讯作者：
Shi W
中科院分区：
文献类型：
--
作者：
Hu W;Yang Z;Lux R;Zhao M;Wang J;He X;Shi W
IV型皮利（TFP）和胞外多糖（EPS）是黄色粘球菌社会行为的重要组成部分，包括滑行运动和子实体形成。虽然已经提出了TFP和EPS之间的特定相互作用，但尚未在自然条件下对这些相互作用进行直接观察。在这项研究中，我们发现，截短的PilA蛋白（PilACt），只含有C-末端结构域（氨基酸32-208）是足够的EPS结合在体外。此外，构建了增强型绿色荧光蛋白（eGFP）与PilACt融合蛋白，并用于标记M. xanthus在激光共聚焦显微镜下，eGFP-PilACt结合的子实体、拖尾结构和生物膜与小麦胚芽凝集素（WGA）标记的EPS结构具有相似的模式。本研究表明，eGFP-PilACt融合蛋白能够有效地标记M. xanthus的，并首次提供了证据的PilA蛋白和EPS在天然条件下的直接相互作用。
Type IV pili (TFP) and exopolysaccharides (EPS) are important components for social behaviors in Myxococcus xanthus, including gliding motility and fruiting body formation. Although specific interactions between TFP and EPS have been proposed, direct observations of these interactions under native condition have not yet been made. In this study, we found that a truncated PilA protein (PilACt) which only contains the C-terminal domain (amino acids 32-208) is sufficient for EPS binding in vitro. Furthermore, an enhanced green fluorescent protein (eGFP) and PilACt fusion protein was constructed and used to label the native EPS in M. xanthus. Under confocal laser scanning microscope, the eGFP-PilACt-bound fruiting bodies, trail structures and biofilms exhibited similar patterns as the wheat germ agglutinin lectin (WGA)-labeled EPS structures. This study showed that eGFP-PilACt fusion protein was able to efficiently label the EPS of M. xanthus and for the first time provided evidence for the direct interaction between the PilA protein and EPS under native conditions.
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影响因子：
3.2
作者：
BEHMLANDER, RM;DWORKIN, M
通讯作者：
DWORKIN, M
影响因子：
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IRVIN, RT;DOIG, P;HODGES, RS
通讯作者：
HODGES, RS
影响因子：
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Li, Q;Lau, A;Stanley, EF
通讯作者：
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作者：
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通讯作者：
DWORKIN, M
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作者：
Craig, L;Taylor, RK;Tainer, JA
通讯作者：
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