Rimbp, a New Marker for the Nervous System of the Tunicate Ciona robusta.
Rimbp, a New Marker for the Nervous System of the Tunicate Ciona robusta.
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DOI：
10.3390/genes11091006
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发表时间：
2020-08-27
期刊：
影响因子：
3.5
通讯作者：
Ristoratore F
中科院分区：
文献类型：
--
作者：
Coppola U;Olivo P;D'Aniello E;Johnson CJ;Stolfi A;Ristoratore F
通过调节突触前活动区神经递质释放的蛋白质建立突触前机制被认为是动物进化的基本步骤。Rab3相互作用分子结合蛋白（Rimbps）是突触前活动区的重要组成部分，也是钙稳态的关键参与者。虽然Rimbp参与这些动态已被描述在遥远的相关模型，如苍蝇和人类，这个家庭在大多数无脊椎动物的作用仍然模糊。为了填补这一空白，我们定义了Rimbp家族在动物中的进化历史，从海绵到哺乳动物。我们报告，第一次，表达的两种异构体的独特的Rimbp家族成员在玻璃海鞘粗壮在不同的领域的幼虫神经系统。我们确定了内含子增强子，能够驱动表达在不同的神经系统领土部分对应于Rimbp内源性表达。Rimbp的基因表达模式的分析和调控元件的鉴定将积极影响我们对玻璃海鞘胚胎发生背景下这个基因家族的理解。
Establishment of presynaptic mechanisms by proteins that regulate neurotransmitter release in the presynaptic active zone is considered a fundamental step in animal evolution. Rab3 interacting molecule-binding proteins (Rimbps) are crucial components of the presynaptic active zone and key players in calcium homeostasis. Although Rimbp involvement in these dynamics has been described in distantly related models such as fly and human, the role of this family in most invertebrates remains obscure. To fill this gap, we defined the evolutionary history of Rimbp family in animals, from sponges to mammals. We report, for the first time, the expression of the two isoforms of the unique Rimbp family member in Ciona robusta in distinct domains of the larval nervous system. We identify intronic enhancers that are able to drive expression in different nervous system territories partially corresponding to Rimbp endogenous expression. The analysis of gene expression patterns and the identification of regulatory elements of Rimbp will positively impact our understanding of this family of genes in the context of Ciona embryogenesis.
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影响因子：
2.7
作者：
D'Aniello, Enrico;Pezzotti, Maria Rosa;Branno, Margherita
通讯作者：
Branno, Margherita
影响因子：
3.7
作者：
Coppola U;Caccavale F;Scelzo M;Holland ND;Ristoratore F;D'Aniello S
通讯作者：
D'Aniello S
DOI：
10.1073/pnas.1605256113
发表时间：
2016-10-11
影响因子：
11.1
作者：
Grauel, M. Katharina;Maglione, Marta;Rosenmund, Christian
通讯作者：
Rosenmund, Christian
影响因子：
30.8
作者：
Aligianis, IA;Johnson, CA;Maher, ER
通讯作者：
Maher, ER
影响因子：
64.8
作者：
Delsuc, F;Brinkmann, H;Philippe, H
通讯作者：
Philippe, H