Does Fine Color Discrimination Learning in Free-Flying Honeybees Change Mushroom-Body Calyx Neuroarchitecture?
Does Fine Color Discrimination Learning in Free-Flying Honeybees Change Mushroom-Body Calyx Neuroarchitecture?
复制标题
DOI：
10.1371/journal.pone.0164386
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Dyer AG
中科院分区：
文献类型：
--
作者：
Sommerlandt FM;Spaethe J;Rössler W;Dyer AG
蜜蜂学习奖励花朵的颜色信息，并在未来的决策中回忆这些记忆。为了进行精细的颜色辨别，蜜蜂需要同时呈现目标刺激和干扰刺激来进行差异调节，以形成长期记忆。在这里，我们研究了颜色信息的长期存储是否塑造了蘑菇体肾盏中小肾小球的神经网络，以及这是否取决于条件反射的类型。自由飞行的蜜蜂被单独训练成一对感知相似的颜色，要么对其中一种颜色进行绝对调节，要么对两种颜色进行差异调节。随后，对任一调节组的蜜蜂进行了两种颜色的无奖励辨别测试。只有经过差异条件训练的蜜蜂才更喜欢之前学到的颜色，而绝对条件训练组和未接受刺激组的蜜蜂则在颜色刺激中随机选择。然后将所有蜜蜂单独放在黑暗中三天，以形成完整的长期记忆。随后使用整体免疫染色来量化蘑菇体唇部和衣领中微球数量和密度的变化。我们发现各组之间的神经纤维体积和小肾小球总数没有显着差异，但绝对调节组的学习成绩与小肾小球密度呈负相关。基于这些发现，我们的目标是推广未来的研究方法，将自由飞行条件下蜜蜂的行为相关颜色学习测试与神经影像分析相结合；我们还讨论了这种方法可能的局限性。
Honeybees learn color information of rewarding flowers and recall these memories in future decisions. For fine color discrimination, bees require differential conditioning with a concurrent presentation of target and distractor stimuli to form a long-term memory. Here we investigated whether the long-term storage of color information shapes the neural network of microglomeruli in the mushroom body calyces and if this depends on the type of conditioning. Free-flying honeybees were individually trained to a pair of perceptually similar colors in either absolute conditioning towards one of the colors or in differential conditioning with both colors. Subsequently, bees of either conditioning groups were tested in non-rewarded discrimination tests with the two colors. Only bees trained with differential conditioning preferred the previously learned color, whereas bees of the absolute conditioning group, and a stimuli-naïve group, chose randomly among color stimuli. All bees were then kept individually for three days in the dark to allow for complete long-term memory formation. Whole-mount immunostaining was subsequently used to quantify variation of microglomeruli number and density in the mushroom-body lip and collar. We found no significant differences among groups in neuropil volumes and total microglomeruli numbers, but learning performance was negatively correlated with microglomeruli density in the absolute conditioning group. Based on these findings we aim to promote future research approaches combining behaviorally relevant color learning tests in honeybees under free-flight conditions with neuroimaging analysis; we also discuss possible limitations of this approach.
登录
查看更多内容
影响因子：
2
作者：
Barth, M;Heisenberg, M
通讯作者：
Heisenberg, M
影响因子：
2.5
作者：
Ehmer, B;Gronenberg, W
通讯作者：
Gronenberg, W
DOI：
10.1007/bf00188925
发表时间：
1992-09-01
影响因子：
2.1
作者：
CHITTKA, L;MENZEL, R
通讯作者：
MENZEL, R
DOI：
10.1080/0005772x.2003.11099566
发表时间：
2003-01-01
期刊：
BEE WORLD
影响因子：
--
作者：
Giurfa, M
通讯作者：
Giurfa, M
影响因子：
2.8
作者：
Dyer, AG;Neumeyer, C;Chittka, L
通讯作者：
Chittka, L