Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement.
Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement.
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DOI：
10.1038/s41587-021-01058-4
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发表时间：
2022-03
影响因子：
46.9
通讯作者：
Wulff BBH
中科院分区：
文献类型：
--
作者：
Gaurav K;Arora S;Silva P;Sánchez-Martín J;Horsnell R;Gao L;Brar GS;Widrig V;John Raupp W;Singh N;Wu S;Kale SM;Chinoy C;Nicholson P;Quiroz-Chávez J;Simmonds J;Hayta S;Smedley MA;Harwood W;Pearce S;Gilbert D;Kangara N;Gardener C;Forner-Martínez M;Liu J;Yu G;Boden SA;Pascucci A;Ghosh S;Hafeez AN;O'Hara T;Waites J;Cheema J;Steuernagel B;Patpour M;Justesen AF;Liu S;Rudd JC;Avni R;Sharon A;Steiner B;Kirana RP;Buerstmayr H;Mehrabi AA;Nasyrova FY;Chayut N;Matny O;Steffenson BJ;Sandhu N;Chhuneja P;Lagudah E;Elkot AF;Tyrrell S;Bian X;Davey RP;Simonsen M;Schauser L;Tiwari VK;Randy Kutcher H;Hucl P;Li A;Liu DC;Mao L;Xu S;Brown-Guedira G;Faris J;Dvorak J;Luo MC;Krasileva K;Lux T;Artmeier S;Mayer KFX;Uauy C;Mascher M;Bentley AR;Keller B;Poland J;Wulff BBH
节节麦(Aegiloptauschii)是面包小麦D亚基因组的二倍体野生祖先，是遗传多样性的储藏库，可用于改良面包小麦的表现和环境适应性。这里我们对242个Ae进行了测序。并将它们与小麦D亚组进行比较，以确定基因组的多样性。我们发现了一个罕见的Ae.在地理上仅限于现在的佐治亚州的tauschii在独立杂交中贡献了小麦D亚基因组，从而产生了现代面包小麦。通过基于k-mer的关联作图，我们确定了含有抗病和抗虫候选基因的离散基因组区域，并通过转基因和远缘杂交证明了它们的功能转移到小麦中，包括建立了一个含有不同Ae的六倍体文库。节节草属基因组。利用Ae的基因组多样性。节节草祖先的二倍体基因组可以在适合繁殖的六倍体背景下快速发现特征和进行功能遗传验证。探索小麦D亚基因组野生祖先的多样性可以快速发现性状。
Aegilops tauschii, the diploid wild progenitor of the D subgenome of bread wheat, is a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. Here we sequenced 242 Ae. tauschii accessions and compared them to the wheat D subgenome to characterize genomic diversity. We found that a rare lineage of Ae. tauschii geographically restricted to present-day Georgia contributed to the wheat D subgenome in the independent hybridizations that gave rise to modern bread wheat. Through k-mer-based association mapping, we identified discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of hexaploids incorporating diverse Ae. tauschii genomes. Exploiting the genomic diversity of the Ae. tauschii ancestral diploid genome permits rapid trait discovery and functional genetic validation in a hexaploid background amenable to breeding. Exploring the diversity of the wild progenitor of the wheat D subgenome allows for rapid trait discovery.
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影响因子：
5.9
作者：
Delorean E;Gao L;Lopez JFC;Open Wild Wheat Consortium;Wulff BBH;Ibba MI;Poland J
通讯作者：
Poland J
影响因子：
3.1
作者：
Dhakal, Smit;Tan, Chor-Tee;Liu, Shuyu
通讯作者：
Liu, Shuyu
影响因子：
2
作者：
Das, Modan K.;Bai, Guihua;Rajaram, Sanjaya
通讯作者：
Rajaram, Sanjaya
DOI：
10.1073/pnas.142284999
发表时间：
2002-07-09
影响因子：
11.1
作者：
Brueggeman, R;Rostoks, N;Kleinhofs, A
通讯作者：
Kleinhofs, A
DOI：
10.1073/pnas.072223799
发表时间：
2002-06-11
影响因子：
11.1
作者：
Huang, S;Sirikhachornkit, A;Gornicki, P
通讯作者：
Gornicki, P