Expansion of CD4+ T cells with a cytotoxic phenotype in patients with B‐chronic lymphocytic leukaemia (B‐CLL)
Expansion of CD4+ T cells with a cytotoxic phenotype in patients with B‐chronic lymphocytic leukaemia (B‐CLL)
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B-慢性淋巴细胞白血病 (B-CLL) 患者中具有细胞毒性表型的 CD4+ T 细胞的扩增
DOI：
10.1046/j.1365-2249.2001.01639.x
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发表时间：
2001
影响因子：
4.6
通讯作者：
P. Lydyard
中科院分区：
文献类型：
--
作者：
N. Porakishvili;T. Roschupkina;T. Kalber;Andrew P. Jewell;K. Patterson;Kwee Yong;P. Lydyard
CD4/CD8比值和T细胞功能异常在B -慢性淋巴细胞白血病（B - CLL）患者中已被证实。我们已经证明含有丝氨酸酯酶和穿孔素（PF）的CD4+ T细胞在这些患者的血液中增加。通过流式细胞术，我们发现CD4+ PF+细胞是CD57+，但缺乏CD28的表达，表明这是一个成熟的群体。CD8+ T细胞中的相同表型是成熟细胞毒性T细胞的特征。然而，与CD8+ T细胞相比，CD4+ T细胞CD45RO阳性比CD45RA阳性更频繁，这表明先前的抗原经历。相反，这个群体缺乏CD69或HLA - DR的表达，认为它们没有被激活或它们是一个异常的T细胞群体。它们的组成细胞因子水平显示它们主要含有il - 4而不含ifn - γ，提示Th2表型。CD4+ PF+ T细胞群的作用目前尚不确定。然而，这种潜在的细胞毒性T细胞群既可以通过产生il - 4来提高B - CLL肿瘤细胞的存活率，也可以独立于药物治疗，导致这种肿瘤患者经常出现的免疫缺陷状态。
Abnormal CD4/CD8 ratios and T‐cell function have previously been shown in patients with B‐chronic lymphocytic leukaemia (B‐CLL). We have demonstrated that CD4+ T cells containing both serine esterase and perforin (PF) are increased in the blood of these patients. Using flow cytometry, we have shown that the CD4+ PF+ cells were CD57+ but lacked expression of CD28, suggesting a mature population. The same phenotype in CD8+ T cells is characteristic of mature cytotoxic T cells. However, in contrast to the CD8+ T cells, the CD4+ T cells were more frequently CD45RO positive than CD45RA positive, indicating prior antigen experience. In contrast, this population lacked expression of either CD69 or HLA‐DR, arguing that they were not activated or that they are an abnormal population of T cells. Their constitutive cytokine levels showed them mainly to contain IL4 and not IFNγ, suggesting a Th2 phenotype. The role of the CD4+ PF+ T‐cell population is at present uncertain. However, this potentially cytotoxic T‐cell population could contribute both to enhancing survival of the B‐CLL tumour cells through production of IL4, and to the immunodeficient state frequently seen in patients with this tumour, independent of drug treatment.
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人类免疫缺陷病毒糖蛋白特异性 CD4 细胞毒性 T 淋巴细胞参与两种类型的细胞毒性：抗原特异性和细胞-细胞融合相关的细胞裂解。
DOI：
10.1089/aid.1997.13.1017
发表时间：
1997
期刊：
AIDS research and human retroviruses.
影响因子：
--
作者：
Ehret,R;Heinkelein,M;Siliciano,RF;Jassoy,C
通讯作者：
Jassoy,C
影响因子：
4.4
作者：
D. Serrano;J. Monteiro;S. L. Allen;J. Kolitz;P. Schulman;S. Lichtman;A. Buchbinder;V. Vinciguerra;N. Chiorazzi;P. Gregersen
通讯作者：
D. Serrano;J. Monteiro;S. L. Allen;J. Kolitz;P. Schulman;S. Lichtman;A. Buchbinder;V. Vinciguerra;N. Chiorazzi;P. Gregersen
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影响因子：
20.3
作者：
Chu,P;Wierda,WG;Kipps,TJ
通讯作者：
Kipps,TJ
细胞粘附和糖基（HNK-1 和寡甘露糖苷）识别在金鱼视网膜顶盖投射再生锐化中的作用。
DOI：
10.1002/(sici)1097-4695(199812)37:4
发表时间：
1998
期刊：
Journal of neurobiology
影响因子：
--
作者：
Schmidt,JT;Schachner,M
通讯作者：
Schachner,M