TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.
TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.
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DOI：
10.1261/rna.058107.116
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Marzluff WF
中科院分区：
文献类型：
--
作者：
Lackey PE;Welch JD;Marzluff WF
复制依赖性组蛋白mRNA的末端是茎环，而不是所有其他细胞mRNA的3′末端的poly（A）尾。加工后，组蛋白mRNA的3′端在茎环后被修剪成3个核苷酸（nt），如果mRNA被3′hExo进一步修剪，则通过添加非模板尿苷来保持该长度。这些mRNA受到细胞周期的严格调控，并且关键的调控步骤是当DNA复制被抑制时组蛋白mRNA的快速降解。组蛋白mRNA降解的第一步是通过3′hExo将2-4 nt消化到茎中并使该中间体尿苷化。mRNA随后被外泌体降解，停滞的中间体被尿苷酸化。负责组蛋白mRNA寡核苷酸化的酶尚未明确鉴定。使用组蛋白mRNA和降解中间体的高通量测序，我们发现TUT 7的敲除减少了3′端的尿苷化以及茎中主要降解中间体的尿苷化。与此相反，TUT 4的敲除并没有改变3′端的尿苷化模式，并且在组蛋白mRNA降解过程中对茎环中的尿苷化有很小的影响。3′hExo的敲除也改变了组蛋白mRNA的尿苷酸化，表明TUT 7和3′hExo在组蛋白mRNA的修剪和尿苷酸化中共同起作用。
The replication-dependent histone mRNAs end in a stem–loop instead of the poly(A) tail present at the 3′ end of all other cellular mRNAs. Following processing, the 3′ end of histone mRNAs is trimmed to 3 nucleotides (nt) after the stem–loop, and this length is maintained by addition of nontemplated uridines if the mRNA is further trimmed by 3′hExo. These mRNAs are tightly cell-cycle regulated, and a critical regulatory step is rapid degradation of the histone mRNAs when DNA replication is inhibited. An initial step in histone mRNA degradation is digestion 2–4 nt into the stem by 3′hExo and uridylation of this intermediate. The mRNA is then subsequently degraded by the exosome, with stalled intermediates being uridylated. The enzyme(s) responsible for oligouridylation of histone mRNAs have not been definitively identified. Using high-throughput sequencing of histone mRNAs and degradation intermediates, we find that knockdown of TUT7 reduces both the uridylation at the 3′ end as well as uridylation of the major degradation intermediate in the stem. In contrast, knockdown of TUT4 did not alter the uridylation pattern at the 3′ end and had a small effect on uridylation in the stem–loop during histone mRNA degradation. Knockdown of 3′hExo also altered the uridylation of histone mRNAs, suggesting that TUT7 and 3′hExo function together in trimming and uridylating histone mRNAs.
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DOI：
10.1073/pnas.1003505107
发表时间：
2010-08-24
影响因子：
11.1
作者：
Hamill, Stephanie;Wolin, Sandra L.;Reinisch, Karin M.
通讯作者：
Reinisch, Karin M.
DOI：
10.1261/rna.042531.113
发表时间：
2014-01
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Lyons SM;Ricciardi AS;Guo AY;Kambach C;Marzluff WF
通讯作者：
Marzluff WF
DOI：
10.1261/rna.053389.115
发表时间：
2015-11
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Brooks L 3rd;Lyons SM;Mahoney JM;Welch JD;Liu Z;Marzluff WF;Whitfield ML
通讯作者：
Whitfield ML
影响因子：
16.8
作者：
Kaygun, H;Marzluff, WF
通讯作者：
Marzluff, WF
影响因子：
56.9
作者：
MOWRY, KL;STEITZ, JA
通讯作者：
STEITZ, JA