Integrating Embryonic Development and Evolutionary History to Characterize Tentacle-Specific Cell Types in a Ctenophore.
Integrating Embryonic Development and Evolutionary History to Characterize Tentacle-Specific Cell Types in a Ctenophore.
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DOI：
10.1093/molbev/msy171
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发表时间：
2018-12-01
影响因子：
10.7
通讯作者：
Ryan JF
中科院分区：
文献类型：
--
作者：
Babonis LS;DeBiasse MB;Francis WR;Christianson LM;Moss AG;Haddock SHD;Martindale MQ;Ryan JF
新性状的起源可以促进扩展到新的生态位并推动物种形成。栉水母（栉水母）的统一之处在于它们拥有一种新的细胞类型：胶母细胞，一种仅在触手中发现的粘附细胞。虽然含有胶原细胞的触须是栉水母捕获猎物的基础，但一些物种的触须缺乏胶原细胞，而另一些物种则完全失去了触须。我们使用来自36个栉水母物种的转录组来鉴定在缺乏胶母细胞和触手的谱系中特异性发生的基因损失。我们将这些胶母细胞特异性和触手特异性候选基因与leidyi记忆藻胚胎发生过程中的时间RNA-Seq交叉对照，发现这两组候选基因在触手形态发生过程中优先表达。我们还从成人触手球的两个数据集中证明了候选蛋白的显著上调。两组候选物都富含n端信号肽和与分泌相关的蛋白质结构域；在候选触手中，我们还鉴定了刺胞毒素蛋白的同源物，提供了诱人的证据，证明栉水母触手可能分泌毒素连同它们的粘合剂。最后，通过细胞谱系追踪，我们证明了胶质母细胞和神经元具有共同的祖细胞，这表明胶质母细胞的进化涉及神经分泌基因调控网络的共同选择。总之，这些数据提供了对栉水母细胞类型多样性的遗传结构的初步一瞥。
The origin of novel traits can promote expansion into new niches and drive speciation. Ctenophores (comb jellies) are unified by their possession of a novel cell type: the colloblast, an adhesive cell found only in the tentacles. Although colloblast-laden tentacles are fundamental for prey capture among ctenophores, some species have tentacles lacking colloblasts and others have lost their tentacles completely. We used transcriptomes from 36 ctenophore species to identify gene losses that occurred specifically in lineages lacking colloblasts and tentacles. We cross-referenced these colloblast- and tentacle-specific candidate genes with temporal RNA-Seq during embryogenesis in Mnemiopsis leidyi and found that both sets of candidates are preferentially expressed during tentacle morphogenesis. We also demonstrate significant upregulation of candidates from both data sets in the tentacle bulb of adults. Both sets of candidates were enriched for an N-terminal signal peptide and protein domains associated with secretion; among tentacle candidates we also identified orthologs of cnidarian toxin proteins, presenting tantalizing evidence that ctenophore tentacles may secrete toxins along with their adhesive. Finally, using cell lineage tracing, we demonstrate that colloblasts and neurons share a common progenitor, suggesting the evolution of colloblasts involved co-option of a neurosecretory gene regulatory network. Together these data offer an initial glimpse into the genetic architecture underlying ctenophore cell-type diversity.
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DOI：
10.1016/s0167-4838(99)00237-x
发表时间：
2000-02-09
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
Anderluh, G;Podlesek, Z;Macek, P
通讯作者：
Macek, P
影响因子：
2.6
作者：
EMSON, RH;WHITFIELD, PJ
通讯作者：
WHITFIELD, PJ
影响因子：
4.6
作者：
Dohrmann M;Wörheide G
通讯作者：
Wörheide G
影响因子：
2.7
作者：
Alie, Alexandre;Leclere, Lucas;Manuel, Michael
通讯作者：
Manuel, Michael
影响因子：
2.7
作者：
Henry, JQ;Martindale, MQ
通讯作者：
Martindale, MQ