Rapid direct analysis in real time (DART) mass spectrometric detection of juvenile hormone III and its terpene precursors.
Rapid direct analysis in real time (DART) mass spectrometric detection of juvenile hormone III and its terpene precursors.
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实时的快速直接分析（DART）对青少年激素III及其萜烯前体的质谱检测。
DOI：
10.1007/s00216-010-4269-4
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发表时间：
2010-12
影响因子：
4.3
通讯作者：
Fernandez, Facundo M.
中科院分区：
文献类型：
--
作者：
Navare, Arti T.;Mayoral, Jaime G.;Nouzova, Marcela;Noriega, Fernando G.;Fernandez, Facundo M.
直接实时分析（DART）是一种基于等离子体的环境电离技术，可以快速电离具有高样品通量的小分子。在这项工作中，DART与正交（oa）飞行时间（TOF）质谱仪耦合，并优化了该系统，用于分析昆虫中重要的激素调节剂，幼年激素（JH） III及其萜类前体，即法尼醇，法尼酸和甲基法尼皂酯。利用实验设计（DOE）全因子模型规划优化实验，以确定DART- tof质谱（MS）对JH III分析灵敏度影响最大的DART变量。优化后的DART-TOF质谱法对萜烯标准具有飞摩尔到亚皮摩尔的检出限，质量精度低于5ppm。最后，研究了在oof质谱仪的第一差分泵浦区用源内碰撞诱导解离（CID）区分两种法尼醇异构体的可能性。DART-MS实现了高通量、灵敏的分析，采集时间从30秒到一分钟不等。据我们所知，这是第一次将DART-MS应用于挥发性或半挥发性昆虫萜类化合物的检测和鉴定，并使用DOE方法优化DART-MS分析程序。
Direct analysis in real time (DART) is a plasma-based ambient ionization technique that enables rapid ionization of small molecules with high sample throughput. In this work, DART was coupled to an orthogonal (oa) time-of-flight (TOF) mass spectrometer and the system was optimized for analyzing a vital hormonal regulator in insects, juvenile hormone (JH) III and its terpene precursors, namely, farnesol, farnesoic acid, and methyl farne-soate. Optimization experiments were planned using design of experiments (DOE) full factorial models to identify the most significant DART variables contributing to JH III analysis sensitivity by DART-TOF mass spectrometry (MS). The optimized DART-TOF MS method had femto-mole to sub-picomole detection limits for terpene standards, along with mass accuracies below 5 ppm. Finally, the possibility of distinguishing between two farnesol isomers by in-source-collision-induced dissociation (CID) in the first differentially pumped region of the oaTOF mass spectrometer was investigated. DART-MS enabled high-throughput, sensitive analysis with acquisition times ranging from 30 s to a minute. To the best of our knowledge, this is the first report on the application of DART-MS to the detection and identification of volatile or semi-volatile insect terpenoids, and on the use of DOE approaches to optimize DART-MS analytical procedures.
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影响因子：
2
作者：
Haefliger, Olivier P.;Jeckelmann, Nicolas
通讯作者：
Jeckelmann, Nicolas
影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
DOI：
10.1016/0020-7322(84)90033-3
发表时间：
1984-01-01
期刊：
INTERNATIONAL JOURNAL OF INSECT MORPHOLOGY & EMBRYOLOGY
影响因子：
--
作者：
LOCOCO, DJ;TOBE, SS
通讯作者：
TOBE, SS
影响因子：
7.4
作者：
Petucci, Chris;Diffendal, Jason;Musselman, Brian
通讯作者：
Musselman, Brian
影响因子：
4.1
作者：
Lee, Hyeunjoo;Finckbeiner, Steven;Attygalle, Athula B.
通讯作者：
Attygalle, Athula B.