Auxin Response Factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum).
Auxin Response Factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum).
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DOI：
10.1371/journal.pone.0193517
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Zouine M
中科院分区：
文献类型：
--
作者：
Bouzroud S;Gouiaa S;Hu N;Bernadac A;Mila I;Bendaou N;Smouni A;Bouzayen M;Zouine M
生存生物量生产和作物产量受到广泛的环境压力的严重制约。脱落酸（阿坝）、乙烯和水杨酸（SA）等植物激素参与了植物对逆境胁迫的反应。相比之下，植物激素生长素在应激反应中的作用迄今为止仍然研究得很少。生长素控制植物生长发育的许多方面，生长素应答因子通过直接结合到生长素应答基因的启动子上，在转录激活或抑制生长素应答基因中起关键作用。为了更深入地了解生长素参与番茄生物和非生物胁迫响应的机制，本研究揭示了SlARF基因在生物和非生物胁迫下的表达模式。通过对公共TomExpress网络平台提供的RNAseq数据进行计算机挖掘，确定了几种SlARF对细菌和病毒诱导的各种病原体感染有反应。因此，序列分析显示这些SlARF的5'调控区富含生物和非生物胁迫响应顺式元件。此外，定量qPCR表达分析表明，许多SlARF在盐，干旱和洪水胁迫条件下，番茄叶片和根的差异表达。定量qPCR表达研究进一步指出了SlARF在应激反应中的假定作用，确定了一些miRNA前体可能参与调节暴露于盐和干旱胁迫的根中的SlARF靶基因。这些数据表明，在应激条件下，在转录后水平的SlARF的主动调节。基于几个SlARF基因的转录积累的实质性变化，在这项工作中提出的数据强烈支持生长素参与胁迫反应，从而能够确定一组候选SlARF作为生物和非生物胁迫反应的潜在介质。
Survival biomass production and crop yield are heavily constrained by a wide range of environmental stresses. Several phytohormones among which abscisic acid (ABA), ethylene and salicylic acid (SA) are known to mediate plant responses to these stresses. By contrast, the role of the plant hormone auxin in stress responses remains so far poorly studied. Auxin controls many aspects of plant growth and development, and Auxin Response Factors play a key role in the transcriptional activation or repression of auxin-responsive genes through direct binding to their promoters. As a mean to gain more insight on auxin involvement in a set of biotic and abiotic stress responses in tomato, the present study uncovers the expression pattern of SlARF genes in tomato plants subjected to biotic and abiotic stresses. In silico mining of the RNAseq data available through the public TomExpress web platform, identified several SlARFs as responsive to various pathogen infections induced by bacteria and viruses. Accordingly, sequence analysis revealed that 5’ regulatory regions of these SlARFs are enriched in biotic and abiotic stress-responsive cis-elements. Moreover, quantitative qPCR expression analysis revealed that many SlARFs were differentially expressed in tomato leaves and roots under salt, drought and flooding stress conditions. Further pointing to the putative role of SlARFs in stress responses, quantitative qPCR expression studies identified some miRNA precursors as potentially involved in the regulation of their SlARF target genes in roots exposed to salt and drought stresses. These data suggest an active regulation of SlARFs at the post-transcriptional level under stress conditions. Based on the substantial change in the transcript accumulation of several SlARF genes, the data presented in this work strongly support the involvement of auxin in stress responses thus enabling to identify a set of candidate SlARFs as potential mediators of biotic and abiotic stress responses.
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